Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

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Anti-SIRT2 antibody [EPR20411-105] (ab211033)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20411-105] to SIRT2
Suitable for: WB, IHC-P, IHC-Fr, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-SIRT2 antibody [EPR20411-105]
See all SIRT2 primary antibodies
Description

Rabbit monoclonal [EPR20411-105] to SIRT2
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal brain and brain cortex lysates; Mouse brain and cerebral cortex lysates; Rat brain lysate; MCF7 whole cell lysate. IHC-P: Human, mouse and rat cerebrum tissues. IHC-Fr: Mouse striatum tissue. IP: Human fetal brain lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20411-105
Isotype

IgG
Research areas

Western blot - Anti-SIRT2 antibody [EPR20411-105] (ab211033)

Lane 1 : Anti-SIRT2 antibody [EPR20411-105] (ab211033) at 1/5000 dilution
Lane 2 : Anti-SIRT2 antibody [EPR20411-105] (ab211033) at 1/2000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human brain cortex lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 43 kDa
Observed band size: 36,43 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1: 8 seconds; Lane 2: 1 second.

SIRT2 exists in two main isoforms, SIRT2.1 (43 kDa) and SIRT2.2 (39 kDa). The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:21791548).
Immunohistochemistry (Frozen sections) - Anti-SIRT2 antibody [EPR20411-105] (ab211033)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse striatum tissue labeling SIRT2 with ab211033 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on mouse stratium is observed [PMID: 17447894]. The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT2 antibody [EPR20411-105] (ab211033)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling SIRT2 with ab211033 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on myelinated axons of human cerebrum is observed [PMID: 17447894]. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-SIRT2 antibody [EPR20411-105] (ab211033)

SIRT2 was immunoprecipitated from 0.35 mg of Human fetal brain lysate with ab211033 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab211033 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: Human fetal brain lysate, 10 μg (Input).

Lane 2: ab211033 IP in Human fetal brain lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211033 in Human fetal brain lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second.
Western blot - Anti-SIRT2 antibody [EPR20411-105] (ab211033)

Anti-SIRT2 antibody [EPR20411-105] (ab211033) at 1/2000 dilution + MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 43 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-SIRT2 antibody [EPR20411-105] (ab211033)

All lanes : Anti-SIRT2 antibody [EPR20411-105] (ab211033) at 1/2000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse cerebral cortex lysate
Lane 3 : Rat brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 43 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1: 3 minutes; Lane 2/3: 5 seconds.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:21791548).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT2 antibody [EPR20411-105] (ab211033)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling SIRT2 with ab211033 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on myelinated axons of mouse cerebrum is observed [PMID: 17447894]. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT2 antibody [EPR20411-105] (ab211033)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling SIRT2 with ab211033 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on myelinated axons of rat cerebrum is observed [PMID: 17447894]. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-SIRT2 antibody [EPR20411-105] (ab211033)

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