Anti-SIRT2 antibody [EPR1667] (ab134171)
![Anti-SIRT2 antibody [EPR1667] (ab134171)](https://www.abcam.com/ps/products/134/ab134171/Images/ab134171-275430-anti-sirt2-antibody-epr1667-western-blot.jpg "Anti-SIRT2 antibody [EPR1667] (ab134171)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1667] to SIRT2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-SIRT2 antibody [EPR1667]
See all SIRT2 primary antibodies -
Description
Rabbit monoclonal [EPR1667] to SIRT2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to residues on the N terminus of Human SIRT2 (UniProt Q8IXJ6).
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Positive control
- SKBR3 and SH-SY5Y cell lysates
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General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR1667 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SIRT2 antibody [EPR1667] (ab134171)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: SIRT2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: SH-SY5Y whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab134171 observed at 48 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab134171 was shown to specifically react with SIRT2 in wild-type HAP1 cells. No band was observed when SIRT2 knockout samples were examined. Wild-type and SIRT2 knockout samples were subjected to SDS-PAGE. Ab134171 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-SIRT2 antibody [EPR1667] (ab134171)](https://www.abcam.com/ps/products/134/ab134171/Images/ab134171-148480-anti-sirt2-antibody-epr1667-western-blot.jpg)
Western blot - Anti-SIRT2 antibody [EPR1667] (ab134171)All lanes : Anti-SIRT2 antibody [EPR1667] (ab134171) at 1/500 dilution
Lane 1 : SKBR3 cell lysate
Lane 2 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 43 kDa
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![Anti-SIRT2 antibody [EPR1667] (ab134171)](https://www.abcam.com/ps/products/134/ab134171/Images/ab134171-3-benefits-of-recombinant-antibodies.png)
Anti-SIRT2 antibody [EPR1667] (ab134171)
