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Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (ab256127)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [123] to Ctip1/BCL-11A - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free
    See all Ctip1/BCL-11A primary antibodies

  • Description

    Mouse monoclonal [123] to Ctip1/BCL-11A - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF,IP or WB

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human Ctip1/BCL-11A aa 600 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q9H165

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human tonsil and breast carcinoma tissue; mouse cerebrum tissue; rat spleen tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    ab256127 is the carrier-free version of ab119275. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256127 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (ab256127)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (ab256127)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling Ctip1/BCL-11A with ab119275 at 5.26, followed by a ready to use secondary antibody. Nuclear staining on human breast carcinoma is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use secondary antibody.

    The section was incubated with ab119275 for 30 mins at room temperature.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab119275).  

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (ab256127)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (ab256127)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Ctip1/BCL-11A with ab119275 at 0.526µg/ml, followed by a ready to use secondary antibody. Nuclear staining on mouse cerebrum is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use secondary antibody.

    The section was incubated with ab119275 for 30 mins at room temperature.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab119275).  

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (ab256127)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (ab256127)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Ctip1/BCL-11A with ab119275 at 5.26µg/ml, followed by a ready to use secondary antibody. Nuclear staining on human tonsil is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use secondary antibody.

    The section was incubated with ab119275 for 30 mins at room temperature.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab119275).  

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (ab256127)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (ab256127)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Ctip1/BCL-11A with ab119275 at 0.526µg/ml, followed by a ready to use secondary antibody. Nuclear staining on rat spleen is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use secondary antibody.

    The section was incubated with ab119275 for 30 mins at room temperature.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab119275).  

  • Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (ab256127)
    Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (ab256127)

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