Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR073G] to Ctip1/BCL-11A - BSA free
Suitable for: IP, WB
Reacts with: Mouse, Human

Product name

Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free
See all Ctip1/BCL-11A primary antibodies
Description

Rabbit monoclonal [BLR073G] to Ctip1/BCL-11A - BSA free
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide within Human Ctip1/BCL-11A aa 1-50. The exact sequence is proprietary.
Database link: Q9H165
Positive control
- WB: Ramos, A20, CH27, HEK-293T, Jurkat, MOLT4 and Raji whole cell lysate. IP: Ramos whole cell lysate.
General notes

This product is sold under License from Bethyl Laboratories, Inc

Application	Species
IP	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

BLR073G
Isotype

IgG
Research areas

Western blot - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423)

All lanes : Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423) at 1/1000 dilution

Lane 1 : Ramos whole cell lysate
Lane 2 : HEK-293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : MOLT4 whole cell lysate
Lane 5 : U-2 OS whole cell lysate
Lane 6 : Raji whole cell lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG

Exposure time: 3 minutes

Cells prepared using NETN lysis buffer.
Western blot - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423)

All lanes : Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423) at 1/1000 dilution

Lane 1 : BW5147.3 whole cell lysate
Lane 2 : A20 whole cell lysate
Lane 3 : RenCa whole cell lysate
Lane 4 : EL4 whole cell lysate
Lane 5 : CH27 whole cell lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG

Exposure time: 3 minutes

Cells prepared using NETN lysis buffer.
Immunoprecipitation - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423)

Ctip1/BCL-11A was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell lysate with ab264423 at 6 µl/mg lysate. Western blot was performed from the immunoprecipitate using ab264423 at 1/1000 dilution.

Lane 1: ab264423 IP in Ramos whole cell lysate.
Lane 2: Control IgG.

Exposure time: 75 secs.
Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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