Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR073G] to Ctip1/BCL-11A - BSA free
- Suitable for: IP, WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free
See all Ctip1/BCL-11A primary antibodies -
Description
Rabbit monoclonal [BLR073G] to Ctip1/BCL-11A - BSA free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman -
Immunogen
Synthetic peptide within Human Ctip1/BCL-11A aa 1-50. The exact sequence is proprietary.
Database link: Q9H165 -
Positive control
- WB: Ramos, A20, CH27, HEK-293T, Jurkat, MOLT4 and Raji whole cell lysate. IP: Ramos whole cell lysate.
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General notes
This product is sold under License from Bethyl Laboratories, Inc
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
BLR073G -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423) at 1/1000 dilution
Lane 1 : Ramos whole cell lysate
Lane 2 : HEK-293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : MOLT4 whole cell lysate
Lane 5 : U-2 OS whole cell lysate
Lane 6 : Raji whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Exposure time: 3 minutesCells prepared using NETN lysis buffer.
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All lanes : Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423) at 1/1000 dilution
Lane 1 : BW5147.3 whole cell lysate
Lane 2 : A20 whole cell lysate
Lane 3 : RenCa whole cell lysate
Lane 4 : EL4 whole cell lysate
Lane 5 : CH27 whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Exposure time: 3 minutesCells prepared using NETN lysis buffer.
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Ctip1/BCL-11A was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell lysate with ab264423 at 6 µl/mg lysate. Western blot was performed from the immunoprecipitate using ab264423 at 1/1000 dilution.
Lane 1: ab264423 IP in Ramos whole cell lysate.
Lane 2: Control IgG.Exposure time: 75 secs.
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