Anti-SIRT2 antibody [EP1668Y] (ab51023)
![Anti-SIRT2 antibody [EP1668Y] (ab51023)](https://www.abcam.com/ps/products/51/ab51023/Images/ab51023-62859-anti-sirt2-antibody-ep1668y-western-blot.jpg "Anti-SIRT2 antibody [EP1668Y] (ab51023)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1668Y] to SIRT2
- Suitable for: WB, IP, Flow Cyt, ICC/IF
- Reacts with: Rat, Human
Overview
-
Product name
Anti-SIRT2 antibody [EP1668Y]
See all SIRT2 primary antibodies -
Description
Rabbit monoclonal [EP1668Y] to SIRT2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF RatIP HumanWB Human
-
Immunogen
Synthetic peptide within Human SIRT2 (C terminal). The exact sequence is proprietary.
-
Positive control
- WB: SKBR-3 cell lysate ICC/IF: PC 12 cells Flow Cyt: HeLa cells IP: HeLa cell lysate
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1668Y -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-SIRT2 antibody [EP1668Y] (ab51023)Anti-SIRT2 antibody [EP1668Y] (ab51023) at 1/1000 dilution + SKBR-3 cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 43 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
-
![Immunocytochemistry/ Immunofluorescence - Anti-SIRT2 antibody [EP1668Y] (ab51023)](https://www.abcam.com/ps/products/51/ab51023/Images/ab51023-255430-anti-sirt2-antibody-ep1668y-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SIRT2 antibody [EP1668Y] (ab51023)Immunofluorescence staining of PC-12 cells using ab51023 (green) at a dilution of 1/250. The cells were fixed in 4% PFA and counter stained with an anti-tubulin antibody (red) and DAPI (blue).
-
![Immunoprecipitation - Anti-SIRT2 antibody [EP1668Y] (ab51023)](https://www.abcam.com/ps/products/51/ab51023/Images/ab51023-6-anti-sirt2-antibody-ep1668y-immunoprecipitation-hela-human.jpg)
Immunoprecipitation - Anti-SIRT2 antibody [EP1668Y] (ab51023)Purified ab51023 at 1/50 dilution (2µg) immunoprecipitating SIRT2 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab51023 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51023 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 36-43 kDa -
![Flow Cytometry - Anti-SIRT2 antibody [EP1668Y] (ab51023)](https://www.abcam.com/ps/products/51/ab51023/Images/ab51023-62857-anti-sirt2-antibody-ep1668y-flow-cytometry.jpg)
Flow Cytometry - Anti-SIRT2 antibody [EP1668Y] (ab51023)Flow cytometry analysis of permeabilized Hela cells using anti-SIR2/SIRT2 (red)(ab51023) or a rabbit IgG (negative)(green). -
![Anti-SIRT2 antibody [EP1668Y] (ab51023)](https://www.abcam.com/ps/products/51/ab51023/Images/ab51023-5-benefits-of-recombinant-antibodies.png)
Anti-SIRT2 antibody [EP1668Y] (ab51023)
