Antibodies, Proteins, Kits and Reagents for Life Science

341 740 ₸ Product size

100 µl 341 740 ₸ 10 µl 127 315 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1668Y] to SIRT2
Suitable for: WB, IP, Flow Cyt, ICC/IF
Reacts with: Rat, Human

Product name

Anti-SIRT2 antibody [EP1668Y]
See all SIRT2 primary antibodies
Description

Rabbit monoclonal [EP1668Y] to SIRT2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Rat
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human SIRT2 (C terminal). The exact sequence is proprietary.
Positive control
- WB: SKBR-3 cell lysate ICC/IF: PC 12 cells Flow Cyt: HeLa cells IP: HeLa cell lysate
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP1668Y
Isotype

IgG
Research areas

Western blot - Anti-SIRT2 antibody [EP1668Y] (ab51023)

Anti-SIRT2 antibody [EP1668Y] (ab51023) at 1/1000 dilution + SKBR-3 cell lysate at 10 µg

Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution

Predicted band size: 43 kDa
Observed band size: 36 kDa
why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-SIRT2 antibody [EP1668Y] (ab51023)

Immunofluorescence staining of PC-12 cells using ab51023 (green) at a dilution of 1/250. The cells were fixed in 4% PFA and counter stained with an anti-tubulin antibody (red) and DAPI (blue).
Immunoprecipitation - Anti-SIRT2 antibody [EP1668Y] (ab51023)

Purified ab51023 at 1/50 dilution (2µg) immunoprecipitating SIRT2 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab51023 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51023 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 36-43 kDa
Flow Cytometry - Anti-SIRT2 antibody [EP1668Y] (ab51023)

Flow cytometry analysis of permeabilized Hela cells using anti-SIR2/SIRT2 (red)(ab51023) or a rabbit IgG (negative)(green).
Anti-SIRT2 antibody [EP1668Y] (ab51023)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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