Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CLSTN1 antibody [EPR2963] (ab134130)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2963] to CLSTN1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CLSTN1 antibody [EPR2963]
    See all CLSTN1 primary antibodies

  • Description

    Rabbit monoclonal [EPR2963] to CLSTN1

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 91%  homology with CLSTN2. Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human CLSTN1 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Human fetal brain lysate; 293T cell lysate; U87 MG lysate; SH SY5Y lysate and Neuro 2a lysate

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-CLSTN1 antibody [EPR2963] (ab134130)
    Immunocytochemistry/ Immunofluorescence - Anti-CLSTN1 antibody [EPR2963] (ab134130)
    Immfluorescent staining of paraffin embedded Human SH SY 5Y cells labelling CLSTN1 with ab134130 at 1/100 dilution
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLSTN1 antibody [EPR2963] (ab134130)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLSTN1 antibody [EPR2963] (ab134130)

    Immunohistochemical analysis of paraffin embedded Human brain tissue labelling CLSTN1 with ab134130 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-CLSTN1 antibody [EPR2963] (ab134130)
    Western blot - Anti-CLSTN1 antibody [EPR2963] (ab134130)
    All lanes : Anti-CLSTN1 antibody [EPR2963] (ab134130) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : 293T cell lysate
    Lane 3 : U87 MG lysate
    Lane 4 : SH SY5Y lysate
    Lane 5 : Neuro 2a lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti mouse at 1/2000 dilution

    Predicted band size: 110 kDa

  • Western blot - Anti-CLSTN1 antibody [EPR2963] (ab134130)
    Western blot - Anti-CLSTN1 antibody [EPR2963] (ab134130)
    Anti-CLSTN1 antibody [EPR2963] (ab134130) at 1/200000 dilution + Human fetal brain tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 110 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and diluting buffer: 5% NFDM/TBST

    Extra band at: 90kDa   We are unsure as to the identity of this extra band.

  • Anti-CLSTN1 antibody [EPR2963] (ab134130)
    Anti-CLSTN1 antibody [EPR2963] (ab134130)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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