Anti-14-3-3 zeta (phospho T232) antibody (ab59374)
Key features and details
- Rabbit polyclonal to 14-3-3 zeta (phospho T232)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-14-3-3 zeta (phospho T232) antibody
See all 14-3-3 zeta primary antibodies -
Description
Rabbit polyclonal to 14-3-3 zeta (phospho T232) -
Host species
Rabbit -
Specificity
Detects endogenous levels of 14-3-3 zeta + delta only when phosphorylated at threonine 232. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic phosphopeptide derived from human 14-3-3 zeta + delta around the phosphorylation site of threonine 232 (S-D-TP-Q-G).
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Positive control
- Human brain.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This product was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 zeta (phospho T232) antibody (ab59374)Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab59374 at a 1/50-1/100 dilution.
Left image: untreated.
Right image: treated with immunizing phosphopeptide.
