Anti-14-3-3 zeta (phospho S58) antibody (ab51109)
Key features and details
- Rabbit polyclonal to 14-3-3 zeta (phospho S58)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-14-3-3 zeta (phospho S58) antibody
See all 14-3-3 zeta primary antibodies -
Description
Rabbit polyclonal to 14-3-3 zeta (phospho S58) -
Host species
Rabbit -
Specificity
14-3-3 zeta (phospho S58) antibody detects endogenous levels of 14-3-3 zeta only when phosphorylated at serine 58. The immunogen sequence shows 92% homology with 14-3-3 gamma, eta, and beta/alpha. There may be cross reactivity with these other proteins. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic phosphopeptide from Human 14-3-3-zeta, around the phosphorylation site of serine 58 (R-S-phosphoS-W-R).
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-14-3-3 zeta (phospho S58) antibody (ab51109)All lanes : Anti-14-3-3 zeta (phospho S58) antibody (ab51109) at 1/500 dilution
Lane 1 : NIH/3T3 cell extract (treated with UV for 30 mins) with phosphopeptide immunogen (phospho S58)
Lane 2 : NIH/3T3 cell extract (treated with UV for 30 mins)
Predicted band size: 28 kDa
Observed band size: 28 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 zeta (phospho S58) antibody (ab51109)Ab51109 staining human 14-3-3 zeta in human breast carcinoma tissue in immunohistochemistry using paraffin embedded tissue. -
Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 zeta (phospho S58) antibody (ab51109)ICC/IF image of ab51109 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51109, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
