Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATF2 antibody [E243] - ChIP Grade (ab32160)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E243] to ATF2 - ChIP Grade
  • Suitable for: ChIP, WB, IHC-P, ICC, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-ATF2 antibody [E243] - ChIP Grade
    See all ATF2 primary antibodies

  • Description

    Rabbit monoclonal [E243] to ATF2 - ChIP Grade

  • Host species

    Rabbit

  • Specificity

    This antibody recognises ATF2, but does not cross react with other ATF family members.

  • Tested Applications & Species

    Application Species
    ChIP
    Human
    Flow Cyt
    Human
    ICC
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human ATF2 aa 450-550 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa cell lysate. IHC-P: Breast carcinoma tissue. Flow Cyt: HeLa cells. IP: HeLa cell lysate. ChIP: Jurkat cells. ICC: A549 cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ATF2 antibody [E243] - ChIP Grade (ab32160)
    Western blot - Anti-ATF2 antibody [E243] - ChIP Grade (ab32160)
    Anti-ATF2 antibody [E243] - ChIP Grade (ab32160) at 1/10000 dilution + HeLa cell lysate

    Predicted band size: 54 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 antibody [E243] - ChIP Grade (ab32160)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 antibody [E243] - ChIP Grade (ab32160)

    Ab32160, at a dilution of 1/250, staining ATF2 in paraffin embedded breast carcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry - Anti-ATF2 antibody [E243] - ChIP Grade (ab32160)
    Immunocytochemistry - Anti-ATF2 antibody [E243] - ChIP Grade (ab32160)
    Immunocytochemistry analysis of A549 (human lung carcinoma epithelial cell) labeling ATF2 with purified ab32160 at 1/100 dilution (10 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.10 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
    Negative control: PBS instead of the primary antibody.
  • ChIP - Anti-ATF2 antibody [E243] - ChIP Grade (ab32160)
    ChIP - Anti-ATF2 antibody [E243] - ChIP Grade (ab32160)

    Chromatin was prepared from Jurkat (TPA and Ionomycin treated or not) cells according to the Abcam X-ChIP protocol. Cells were fixed with 1% formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of ab32160 (red), and 20µl of protein A/G sepharose beads slurry (10µl of sepharose A beads + 10µl of sepharose G beads). 5μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

  • Immunoprecipitation - Anti-ATF2 antibody [E243] - ChIP Grade (ab32160)
    Immunoprecipitation - Anti-ATF2 antibody [E243] - ChIP Grade (ab32160)

    ab32160 (purified) at 1/60 dilution (20 µg/mL) immunoprecipitating ATF2 in HeLa whole cell lysate.
    Lane 3 (-): HeLa(Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µgab32160 & HeLa whole cell lysateRabbit monoclonal IgG (ab172730) instead of ab32160 in HeLa whole cell lysate
    For western blotting, ab32160 at 1/1000 dilution (2.284 µg/mL) and
    bbit TureBlot: Anti-Rabbit IgG HRP was used as the secondary antibody at 1/1500 dilution.
    Blocking and diluting buffer: 5% NFDM /TBST .

  • Flow Cytometry - Anti-ATF2 antibody [E243] - ChIP Grade (ab32160)
    Flow Cytometry - Anti-ATF2 antibody [E243] - ChIP Grade (ab32160)

    Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling ATF2 (red) with ab32160 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

  • Anti-ATF2 antibody [E243] - ChIP Grade (ab32160)
    Anti-ATF2 antibody [E243] - ChIP Grade (ab32160)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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