Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Tuesday March 16, 2021

Anti-C4BPA antibody [EPR14781(B)] - C-terminal (ab182140)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14781(B)] to C4BPA - C-terminal
Suitable for: WB, IP
Reacts with: Human

Product name

Anti-C4BPA antibody [EPR14781(B)] - C-terminal
See all C4BPA primary antibodies
Description

Rabbit monoclonal [EPR14781(B)] to C4BPA - C-terminal
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human plasma lysate. Human fetal liver lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR14781(B)
Isotype

IgG
Research areas

Western blot - Anti-C4BPA antibody [EPR14781(B)] - C-terminal (ab182140)

Anti-C4BPA antibody [EPR14781(B)] - C-terminal (ab182140) at 1/10000 dilution + Human plasma lysate at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 67 kDa
Observed band size: 70 kDa
why is the actual band size different from the predicted?
Western blot - Anti-C4BPA antibody [EPR14781(B)] - C-terminal (ab182140)

Anti-C4BPA antibody [EPR14781(B)] - C-terminal (ab182140) at 1/2000 dilution + Human fetal liver lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

Predicted band size: 67 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Immunoprecipitation - Anti-C4BPA antibody [EPR14781(B)] - C-terminal (ab182140)

Western blot analysis of C4BPA in Human plasma immunoprecipitated with ab182140 at 1/60 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody used at 1/1000 dilution.
Anti-C4BPA antibody [EPR14781(B)] - C-terminal (ab182140)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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