Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Wednesday March 10, 2021

Anti-C4BPA antibody [EPR14780] (ab200345)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14780] to C4BPA
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-C4BPA antibody [EPR14780]
See all C4BPA primary antibodies
Description

Rabbit monoclonal [EPR14780] to C4BPA
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human plasma lysate. IHC-P: Human transitional cell carcinoma of bladder tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14780
Isotype

IgG
Research areas

Western blot - Anti-C4BPA antibody [EPR14780] (ab200345)

Anti-C4BPA antibody [EPR14780] (ab200345) at 1/5000 dilution + human plasma lysate at 20 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 67 kDa
Observed band size: 67 kDa

Exposure time: 30 seconds

5% NFDM/TBST: Blocking and diluting buffer.
Western blot - Anti-C4BPA antibody [EPR14780] (ab200345)

All lanes : Anti-C4BPA antibody [EPR14780] (ab200345) at 1/5000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : Human fetalkidney lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 4 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 67 kDa
Observed band size: 67 kDa

Exposure time: 30 seconds

5% NFDM/TBST: Blocking and diluting buffer.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4BPA antibody [EPR14780] (ab200345)

Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling C4BPA using ab200345 at 1/100 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
Inset image: negative control obtained using PBS instead of ab200345 and secondary antibody only.
Note: Plasma staining on human transitional cell carcinoma of bladder tissue was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4BPA antibody [EPR14780] (ab200345)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling C4BPA using ab200345 at 1/100 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
Inset image: negative control obtained using PBS instead of ab200345 and secondary antibody only.
Note: No staining on human skeletal muscle tissue was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-C4BPA antibody [EPR14780] (ab200345)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com