Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-C4BPA antibody [EPR14780] (ab200345)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14780] to C4BPA
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-C4BPA antibody [EPR14780]
    See all C4BPA primary antibodies

  • Description

    Rabbit monoclonal [EPR14780] to C4BPA

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human plasma lysate. IHC-P: Human transitional cell carcinoma of bladder tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14780

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-C4BPA antibody [EPR14780] (ab200345)
    Western blot - Anti-C4BPA antibody [EPR14780] (ab200345)
    Anti-C4BPA antibody [EPR14780] (ab200345) at 1/5000 dilution + human plasma lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 67 kDa
    Observed band size: 67 kDa


    Exposure time: 30 seconds


    5% NFDM/TBST: Blocking and diluting buffer.

  • Western blot - Anti-C4BPA antibody [EPR14780] (ab200345)
    Western blot - Anti-C4BPA antibody [EPR14780] (ab200345)
    All lanes : Anti-C4BPA antibody [EPR14780] (ab200345) at 1/5000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetalkidney lysate
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 4 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 67 kDa
    Observed band size: 67 kDa


    Exposure time: 30 seconds


    5% NFDM/TBST: Blocking and diluting buffer.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4BPA antibody [EPR14780] (ab200345)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4BPA antibody [EPR14780] (ab200345)

    Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling C4BPA using ab200345 at 1/100 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab200345 and secondary antibody only.
    Note: Plasma staining on human transitional cell carcinoma of bladder tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4BPA antibody [EPR14780] (ab200345)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4BPA antibody [EPR14780] (ab200345)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle  tissue labeling C4BPA using ab200345 at 1/100 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab200345 and secondary antibody only.
    Note: No staining on human skeletal muscle tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-C4BPA antibody [EPR14780] (ab200345)
    Anti-C4BPA antibody [EPR14780] (ab200345)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-C4BPA antibody [EPR14780] (ab200345)

© 2021 Astana Biomed Group LLP. All rights reserved.