Anti-Clusterin antibody (ab79280)
Key features and details
- Goat polyclonal to Clusterin
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Clusterin antibody
See all Clusterin primary antibodies -
Description
Goat polyclonal to Clusterin -
Host species
Goat -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to Mouse Clusterin aa 435-448 (C terminal).
Sequence:C-EKALQEYRRKSRAE
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab79280 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Clusterin antibody (ab79280)
HeLa cells stained for Clusterin (green) using ab79280 at 10 µg/ml in ICC/IF. Cells were permeabilized with 0.15% Triton. DAPI was used as a nuclear counterstain.
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Western blot - Anti-Clusterin antibody (ab79280)Anti-Clusterin antibody (ab79280) at 0.3 µg/ml + Human Testis lysate at 35 µg
Predicted band size: 52 kDa
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Western blot - Anti-Clusterin antibody (ab79280)Anti-Clusterin antibody (ab79280) at 0.3 µg/ml + Rat Brain lysate (in RIPA buffer) at 35 µg
Predicted band size: 52 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miyata et al, Circulation. 2001 Sep 18;104(12):1407-12.; PMID: 11560857).
