Anti-C1s antibody (ab66762)
Key features and details
- Rabbit polyclonal to C1s
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-C1s antibody
See all C1s primary antibodies -
Description
Rabbit polyclonal to C1s -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
-
Immunogen
Synthetic peptide corresponding to Human C1s aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab88234) -
Positive control
- Recombinant Human C1s protein (ab116902) can be used as a positive control in WB. This antibody gave a positive signal in Human Plasma Total Protein.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab66762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB HumanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 76 kDa).ICC/IF Use a concentration of 1 µg/ml.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 76 kDa).ICC/IF
Use a concentration of 1 µg/ml.Target
-
Function
C1s B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4. -
Involvement in disease
Defects in C1S are the cause of complement component C1s deficiency (C1SD) [MIM:613783]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 CUB domains.
Contains 1 EGF-like domain.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains. -
Post-translational
modificationsThe iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. - Information by UniProt
-
Database links
- Entrez Gene: 716 Human
- Omim: 120580 Human
- SwissProt: P09871 Human
- Unigene: 458355 Human
-
Alternative names
- Basic proline rich peptide IB 1 antibody
- C1 esterase antibody
- C1S antibody
see all
Images
-
Western blot - Anti-C1s antibody (ab66762)Anti-C1s antibody (ab66762) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
The Complement C1s subcomponent protein contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunocytochemistry/ Immunofluorescence - Anti-C1s antibody (ab66762)ICC/IF image of ab66762 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66762, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HepG2 and MCF7 cells at 1µg/ml.
Protocols
Datasheets and documents
References (0)
ab66762 has not yet been referenced specifically in any publications.
Images
-
Western blot - Anti-C1s antibody (ab66762)Anti-C1s antibody (ab66762) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 76 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
The Complement C1s subcomponent protein contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. -
Immunocytochemistry/ Immunofluorescence - Anti-C1s antibody (ab66762)ICC/IF image of ab66762 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66762, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HepG2 and MCF7 cells at 1µg/ml.
