Antibodies, Proteins, Kits and Reagents for Life Science

345 091 ₸ Product size

100 µl 345 091 ₸ 10 µl 137 366 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Clusterin antibody [EPR2911] (ab92548)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2911] to Clusterin
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Product name

Anti-Clusterin antibody [EPR2911]
See all Clusterin primary antibodies
Description

Rabbit monoclonal [EPR2911] to Clusterin
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human Clusterin aa 400-500. The exact sequence is proprietary.
Positive control
- IHC-P: human breast carcinoma and testis tissue; WB: human testis, brain, tonsil, fetal brain and plasma lysates.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR2911
Isotype

IgG
Research areas

Western blot - Anti-Clusterin antibody [EPR2911] (ab92548)

Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Clusterin knockout HAP1 whole cell lysate (20 µg)
Lane 3: U87-MG whole cell lysate (20 µg)

Lanes 1 - 3: Merged signal (red and green). Green - ab92548 observed at 68 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab92548 was shown to recognize Clusterin in wild-type HAP1 cells as signal was lost at the expected MW in Clusterin knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Clusterin knockout samples were subjected to SDS-PAGE. Ab92548 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] (ab92548)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling Clusterin with purified ab92548 at 1/450 dilution (0.23 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-Clusterin antibody [EPR2911] (ab92548)

All lanes : Anti-Clusterin antibody [EPR2911] (ab92548) at 1/1000 dilution (Purified)

Lane 1 : Human testis lysates
Lane 2 : Human brain lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 52 kDa
Observed band size: 37,68 kDa why is the actual band size different from the predicted?

Clusterin precursor could be cleaved to produce an N-terminal Î±-chain and a C-terminal Î²-chain as was described by PMID: 25402950. ab92548 recognizes α-chain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Clusterin antibody [EPR2911] (ab92548)

ab92548 (unpurified) at 1/100 dilution staining Apolipoprotein J in Human testis tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Western blot - Anti-Clusterin antibody [EPR2911] (ab92548)

All lanes : Anti-Clusterin antibody [EPR2911] (ab92548) at 1/1000 dilution (unpurified)

Lane 1 : Human tonsil tissue lysate
Lane 2 : Fetal brain tissue lysate
Lane 3 : Human Plasma lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 52 kDa
Anti-Clusterin antibody [EPR2911] (ab92548)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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