Anti-KCTD21 antibody [EPR12873(2)] (ab192259)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12873(2)] to KCTD21
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-KCTD21 antibody [EPR12873(2)]
See all KCTD21 primary antibodies -
Description
Rabbit monoclonal [EPR12873(2)] to KCTD21 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB MouseHuman -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain lysate. Mouse and rat brain lysate. Caco-2, C6, RAW264.7, PC-12 and NIH/3T3 cell lysate. Caco-2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12873(2) -
Isotype
IgG -
Research areas
Images
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Anti-KCTD21 antibody [EPR12873(2)] (ab192259) at 1/1000 dilution + human fetal brain lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 30 kDa
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Anti-KCTD21 antibody [EPR12873(2)] (ab192259) at 1/1000 dilution + Caco-2 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 30 kDa
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All lanes : Anti-KCTD21 antibody [EPR12873(2)] (ab192259) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : C6 cell lysate
Lane 4 : RAW264.7 cell lysate
Lane 5 : PC-12 cell lysate
Lane 6 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 30 kDa
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Flow Cytometry analysis of Caco-2 (Human colorectal adenocarcinoma cell line) cells labeling KCTD21 using ab192259 at a 1/220 dilution (pink). Goat anti-rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
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