Anti-XIAP antibody (ab2541)
Key features and details
- Rabbit polyclonal to XIAP
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-XIAP antibody
See all XIAP primary antibodies -
Description
Rabbit polyclonal to XIAP -
Host species
Rabbit -
Specificity
This antibody detects the 53 kDa human XIAP protein. It does not cross-react with other apoptosis inhibitors. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Available on request. (Peptide available as ab38275.)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 30% Glycerol, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-XIAP antibody (ab2541) This image is courtesy of an Abreview submitted by Dr Kirk McManusab2541 (1/200) staining XIAP in HeLa cells (green). Cells were permeabilised with 0.5% Triton-X100/ PBS, fixed in methanol (-20oC, 10min) and counterstained with DAPI (red) in order to highlight the nucleus. Please refer to abreview for further experimental details.
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Western blot - Anti-XIAP antibody (ab2541)All lanes : Anti-XIAP antibody (ab2541) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 12 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-XIAP antibody (ab2541)ab2541 staining XIAP in MCF7 cells treated with forskolin (ab120058), by ICC/IF. Increase in XIAP expression correlates with increased concentration of forskolin, as described in literature.
The cells were incubated at 37°C for 2h in media containing different concentrations of ab120058 (forskolin) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab2541 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
