MSH2 KO HAP1 (MSH2 knockout Human chronic myelogenous leukemia near-haploid cell line, Left) / WT HAP1 (Right) cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol*.  The cells were incubated with the primary antibody, ab212188 at a 1:6000 dilution (0.01 µg) (red line).  The secondary antibody used was Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at a 1:2000 dilution. The isotype control (black line) antibody was Rabbit monoclonal IgG (ab172730).  An unlabelled control without incubation with primary antibody and secondary antibody was also performed (blue line).

* 90% methanol permeabilisation is recommended. Avoid using TritonX-100 as it may cause non-specific staining.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212188).

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling MSH2 with ab212188 at 1/10000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in human testis was observed (PMID: 10029069). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212188).

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling MSH2 with ab212188 at 1/10000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining only in the stromal cells of human colon cancer; no staining observed in the tumor cells (PMID: 24710284). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212188).

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling MSH2 with ab212188 at 1/10000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in mouse testis was observed (PMID: 10029069). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212188).

Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling MSH2 with ab212188 at 1/10000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining was mainly observed in the spermatogonia in rat testis (PMID: 10029069). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212188).

Immunofluorescent analysis of 100% methanol-fixed A-375 (human malignant melanoma cell line) cells labeling MSH2 with ab212188 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining in A-375 cell line is shown.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212188).

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized A-375 (human malignant melanoma cell line) cell line labeling MSH2 with ab212188 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212188).