Anti-MSH2 antibody [EPR3944] - BSA and Azide free (ab248480)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3944] to MSH2 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-MSH2 antibody [EPR3944] - BSA and Azide free
See all MSH2 primary antibodies -
Description
Rabbit monoclonal [EPR3944] to MSH2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248480 is the carrier-free version of ab133330 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab248480 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
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Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR3944 -
Isotype
IgG -
Research areas
Images
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This data was developed using ab133330, the same antibody clone in a different buffer formulation.
Lane 1 Wild-type HAP1 cell lysate (20 µg)
Lane 2 MSH2 knockout HAP1 cell lysate (20 µg)
Lane 3 HeLa cell lysate (20 µg)
Lane 4 A375 cell lysate (20 µg)
Lanes 1 - 4 Merged signal (red and green). Green - ab133330 observed at 115 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab133330 was shown to recognize MSH2 when MSH2 knockout samples were used, along with additional cross-reactive bands. Wild-type and MSH2 knockout samples were subjected to SDS-PAGE. ab133330 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
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