Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MSH2 antibody [EPR21017-2] (ab212188)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21017-2] to MSH2
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MSH2 antibody [EPR21017-2]
    See all MSH2 primary antibodies

  • Description

    Rabbit monoclonal [EPR21017-2] to MSH2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human MSH2 aa 500 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P43246

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HeLa, A-375, C6 and RAW 264.7 whole cell lysates; Human tonsil, fetal heart and fetal kidney lysates; Rat testis lysate. IHC-P: Human testis and colon cancer tissues; Mouse and rat testis tissues. ICC/IF: A-375 cells. Flow Cyt: A-375 cells; WT HAP1

  • General notes

    To see more of the key markers and tools you need to study the hallmarks of cancer, including genome instability and mutation, please visit the following page.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-MSH2 antibody [EPR21017-2] (ab212188)
    Flow Cytometry - Anti-MSH2 antibody [EPR21017-2] (ab212188)

    MSH2 KO HAP1 (MSH2 knockout Human chronic myelogenous leukemia near-haploid cell line, Left) / WT HAP1 (Right) cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol*.  The cells were incubated with the primary antibody, ab212188 at a 1:6000 dilution (0.01 µg) (red line).  The secondary antibody used was Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at a 1:2000 dilution. The isotype control (black line) antibody was Rabbit monoclonal IgG (ab172730).  An unlabelled control without incubation with primary antibody and secondary antibody was also performed (blue line).

    * 90% methanol permeabilisation is recommended. Avoid using TritonX-100 as it may cause non-specific staining.

  • Western blot - Anti-MSH2 antibody [EPR21017-2] (ab212188)
    Western blot - Anti-MSH2 antibody [EPR21017-2] (ab212188)
    All lanes : Anti-MSH2 antibody [EPR21017-2] (ab212188) at 1/1000 dilution

    Lane 1 : Rat testis lysate at 20 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
    Lane 3 : Human tonsil lysate at 10 µg
    Lane 4 : A-375 (human malignant melanoma cell line) whole cell lysate at 10 µg
    Lane 5 : C6 (rat glial tumor cell line) whole cell lysate at 10 µg
    Lane 6 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
    Lane 7 : Human fetal heart lysate at 10 µg
    Lane 8 : Human fetal kidney lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 104 kDa
    Observed band size: 104 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [EPR21017-2] (ab212188)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [EPR21017-2] (ab212188)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling MSH2 with ab212188 at 1/10000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in human testis was observed (PMID: 10029069). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunocytochemistry/ Immunofluorescence - Anti-MSH2 antibody [EPR21017-2] (ab212188)
    Immunocytochemistry/ Immunofluorescence - Anti-MSH2 antibody [EPR21017-2] (ab212188)

    Immunofluorescent analysis of 100% methanol-fixed A-375 (human malignant melanoma cell line) cells labeling MSH2 with ab212188 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining in A-375 cell line is shown.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Flow Cytometry - Anti-MSH2 antibody [EPR21017-2] (ab212188)
    Flow Cytometry - Anti-MSH2 antibody [EPR21017-2] (ab212188)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized A-375 (human malignant melanoma cell line) cell line labeling MSH2 with ab212188 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [EPR21017-2] (ab212188)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [EPR21017-2] (ab212188)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling MSH2 with ab212188 at 1/10000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining only in the stromal cells of human colon cancer; no staining observed in the tumor cells (PMID: 24710284). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [EPR21017-2] (ab212188)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [EPR21017-2] (ab212188)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling MSH2 with ab212188 at 1/10000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in mouse testis was observed (PMID: 10029069). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [EPR21017-2] (ab212188)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [EPR21017-2] (ab212188)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling MSH2 with ab212188 at 1/10000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining was mainly observed in the spermatogonia in rat testis (PMID: 10029069). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-MSH2 antibody [EPR21017-2] (ab212188)
    Anti-MSH2 antibody [EPR21017-2] (ab212188)

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