Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant Human SIT protein (ab151377)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag C-Terminus, His tag N-Terminus
  • Suitable for: SDS-PAGE, HPLC

Description

  • Product name

    Recombinant Human SIT protein
    See all SIT proteins and peptides

  • Purity

    > 95 % SDS-PAGE.
    Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Accession

    Q9Y3P8

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MGSSHHHHHHSSGLVPRGSHMQWTRGRSRSHPGQGRSGESVEEVPLYGNL HYLQTGRLSQDPEPDQQDPTLGGPARAAEEVMCYTSLQLRPPQGRIPGPG TPVKYSEVVLDSEPKSQASGPEPELYASVCAQTRRARASFPDQAYANSQP AASLEHHHHHH

    • Amino acids

      65 to 196

    • Tags

      His tag C-Terminus , His tag N-Terminus

    • Specifications

    Our Abpromise guarantee covers the use of ab151377 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      HPLC

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

      pH: 8.00
      Constituents: 0.24% Tris, 0.06% EDTA, 1.45% Sodium chloride

    • Reconstitution
      Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in 1X PBS.

    General Info

    • Alternative names

      • gp30/40
      • SHP intracting transmembrane adapter protein
      • SHP2-interacting transmembrane adapter protein
      • Signaling threshold-regulating transmembrane adapter 1
      • SIT
      • Sit1
      • SIT1_HUMAN
      • Suppression inducing transmembrane adapter 1
      • Suppression-inducing transmembrane adapter 1
      see all

    • Function

      Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells. Involved in positive selection of T-cells.

    • Tissue specificity

      Specifically expressed in T- and B-cells. Present in plasma cells but not in germinal center B-cells (at protein level). Expressed in T- and B-cell lymphoma.

    • Post-translational
      modifications

      Phosphorylated on tyrosines by LCK, FYN or ZAP70 upon TCR activation; which leads to the recruitment of PTPN11, GRB2 and CSK.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab151377? Please let us know so that we can cite the reference in this datasheet.

ab151377 has not yet been referenced specifically in any publications.

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

    pH: 8.00
    Constituents: 0.24% Tris, 0.06% EDTA, 1.45% Sodium chloride

  • Reconstitution
    Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in 1X PBS.

Images

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