Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MSH2 antibody [EPR21017-2] - BSA and Azide free (ab228325)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21017-2] to MSH2 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MSH2 antibody [EPR21017-2] - BSA and Azide free
    See all MSH2 primary antibodies

  • Description

    Rabbit monoclonal [EPR21017-2] to MSH2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human MSH2 aa 500 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P43246

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human testis tissue. Flow Cyt: WT HAP1

  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®). 

    ab228325 is a PBS-only buffer format of ab212188. Please refer to ab212188 for recommended dilutions, protocols, and image data.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Flow Cytometry - Anti-MSH2 antibody [EPR21017-2EPR21017-2] - BSA and Azide free (ab228325)
    Flow Cytometry - Anti-MSH2 antibody [EPR21017-2EPR21017-2] - BSA and Azide free (ab228325)

    MSH2 KO HAP1 (MSH2 knockout Human chronic myelogenous leukemia near-haploid cell line, Left) / WT HAP1 (Right) cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol*.  The cells were incubated with the primary antibody, ab212188 at a 1:6000 dilution (0.01 µg) (red line).  The secondary antibody used was Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at a 1:2000 dilution. The isotype control (black line) antibody was Rabbit monoclonal IgG (ab172730).  An unlabelled control without incubation with primary antibody and secondary antibody was also performed (blue line).

    * 90% methanol permeabilisation is recommended. Avoid using TritonX-100 as it may cause non-specific staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212188).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [EPR21017-2] - BSA and Azide free (ab228325)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [EPR21017-2] - BSA and Azide free (ab228325)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling MSH2 with ab212188 at 1/10000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in human testis was observed (PMID: 10029069). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212188).

  • Anti-MSH2 antibody [EPR21017-2] - BSA and Azide free (ab228325)
    Anti-MSH2 antibody [EPR21017-2] - BSA and Azide free (ab228325)

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