Anti-CACNB2 antibody [EPR23419-58] (ab253193)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23419-58] to CACNB2
- Suitable for: WB
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-CACNB2 antibody [EPR23419-58] -
Description
Rabbit monoclonal [EPR23419-58] to CACNB2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse hippocampus and midbrain tissue lysates; Rat hippocampus and brain tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23419-58 -
Isotype
IgG
Images
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All lanes : Anti-CACNB2 antibody [EPR23419-58] (ab253193) at 1/1000 dilution
Lane 1 : Mouse hippocampus tissue lysate
Lane 2 : Mouse midbrain tissue lysate
Lane 3 : Mouse placenta tissue lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rat hippocampus tissue lysate
Lane 6 : Rat placenta tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 73 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 16822546, 24681347).
Negative control: placenta (PMID: 16822546).
Exposure time: Lane 1, 5: 37 seconds Lane 2-3, 4, 6: 114 seconds.
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