Antibodies, Proteins, Kits and Reagents for Life Science

Anti-IFFO1 antibody [EPR22711-61] (ab242130)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22711-61] to IFFO1
Suitable for: WB, IP
Knockout validated
Reacts with: Human

Product name

Anti-IFFO1 antibody [EPR22711-61]
See all IFFO1 primary antibodies
Description

Rabbit monoclonal [EPR22711-61] to IFFO1
Host species

Rabbit
Tested applications

Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt (Intra),ICC or IHC-P
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293T and HDLM-2 whole cell lysates. IP: HEK-293T whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22711-61
Isotype

IgG

Western blot - Anti-IFFO1 antibody [EPR22711-61] (ab242130)

All lanes : Anti-IFFO1 antibody [EPR22711-61] (ab242130) at 1/1000 dilution

Lane 1 : Wild-type HEK-293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : IFFO1 knockout HEK-293T whole cell lysate
Lane 3 : IFFO2 knockout HEK-293T whole cell lysate
Lane 4 : IFFO1/IFFO2 knockout HEK-293T whole cell lysate

Predicted band size: 62 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Data was kindly provided by an anonymous collaborator.

The antibody for IFFO2 is ab242131.
Immunoprecipitation - Anti-IFFO1 antibody [EPR22711-61] (ab242130)

IFFO1 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) whole cell lysate with ab242130 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab242130 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 10 ug

Lane 2: ab242130 IP in HEK-293T whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab242130 in HEK-293T whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 41 seconds
Western blot - Anti-IFFO1 antibody [EPR22711-61] (ab242130)

All lanes : Anti-IFFO1 antibody [EPR22711-61] (ab242130) at 1/1000 dilution

Lane 1 : HEK-293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : HDLM-2 (human hodgkin lymphoma) whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 62 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The observed MW is consistent with what has been described in the literature (PMID: 31548606).

Negative control: HeLa (PMID: 31548606).

Exposure time: 3 minutes
Anti-IFFO1 antibody [EPR22711-61] (ab242130)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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