Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IFFO1 antibody [EPR22711-61] (ab242130)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22711-61] to IFFO1
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-IFFO1 antibody [EPR22711-61]
    See all IFFO1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22711-61] to IFFO1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt (Intra),ICC or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T and HDLM-2 whole cell lysates. IP: HEK-293T whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22711-61

  • Isotype

    IgG

Images

  • Western blot - Anti-IFFO1 antibody [EPR22711-61] (ab242130)
    Western blot - Anti-IFFO1 antibody [EPR22711-61] (ab242130)
    All lanes : Anti-IFFO1 antibody [EPR22711-61] (ab242130) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T (human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : IFFO1 knockout HEK-293T whole cell lysate
    Lane 3 : IFFO2 knockout HEK-293T whole cell lysate
    Lane 4 : IFFO1/IFFO2 knockout HEK-293T whole cell lysate

    Predicted band size: 62 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Data was kindly provided by an anonymous collaborator.

    The antibody for IFFO2 is ab242131.

     

     

  • Immunoprecipitation - Anti-IFFO1 antibody [EPR22711-61] (ab242130)
    Immunoprecipitation - Anti-IFFO1 antibody [EPR22711-61] (ab242130)

    IFFO1 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) whole cell lysate with ab242130 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab242130 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 10 ug

    Lane 2: ab242130 IP in HEK-293T whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab242130 in HEK-293T whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 41 seconds

     

  • Western blot - Anti-IFFO1 antibody [EPR22711-61] (ab242130)
    Western blot - Anti-IFFO1 antibody [EPR22711-61] (ab242130)
    All lanes : Anti-IFFO1 antibody [EPR22711-61] (ab242130) at 1/1000 dilution

    Lane 1 : HEK-293T (human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : HDLM-2 (human hodgkin lymphoma) whole cell lysate
    Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 62 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The observed MW is consistent with what has been described in the literature (PMID: 31548606).

    Negative control: HeLa (PMID: 31548606).

    Exposure time: 3 minutes

     

  • Anti-IFFO1 antibody [EPR22711-61] (ab242130)
    Anti-IFFO1 antibody [EPR22711-61] (ab242130)

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