Anti-ErbB2 / HER2 antibody [ICR12] - BSA and Azide free (ab256130)
![Anti-ErbB2 / HER2 antibody [ICR12] - BSA and Azide free (ab256130)](https://www.abcam.com/ps/products/256/ab256130/Images/ab256130-1-anti-erbb-2-antibody-icr12-immunohistochemistry-human-breast-carcinoma.jpg "Anti-ErbB2 / HER2 antibody [ICR12] - BSA and Azide free (ab256130)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [ICR12] to ErbB2 / HER2 - BSA and Azide free
- Suitable for: IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-ErbB2 / HER2 antibody [ICR12] - BSA and Azide free
See all ErbB2 / HER2 primary antibodies -
Description
Rat monoclonal [ICR12] to ErbB2 / HER2 - BSA and Azide free -
Host species
Rat -
Tested applications
Suitable for: IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP or WB -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human ErbB2/ HER2.
Database link: P04626 -
Positive control
- IHC-P: Human breast carcinoma tissue. ICC/IF: SK-BR-3 cells. Flow Cyt: SK-BR-3 cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab256130 is the carrier-free version of ab11710. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
ICR12 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [ICR12] - BSA and Azide free (ab256130)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling ErbB2 / HER2 with ab11710 at 1/400 dilution followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Membranous staining on human breast carcinoma. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide ab11710.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [ICR12] - BSA and Azide free (ab256130)](https://www.abcam.com/ps/products/256/ab256130/Images/ab256130-2-anti-erbb-2-antibody-icr12-immunohistochemistry-human-breast-carcinoma1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 antibody [ICR12] - BSA and Azide free (ab256130)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling ErbB2 / HER2 with ab11710 at 1/400 dilution followed by ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). No staining on human breast carcinoma without expression of HER2 is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide ab11710.
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![Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [ICR12] - BSA and Azide free (ab256130)](https://www.abcam.com/ps/products/256/ab256130/Images/ab256130-3-anti-erbb-2-antibody-icr12-immunocytochemistry-skbr3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [ICR12] - BSA and Azide free (ab256130)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-BR-3 cells labelling EErbB2 / HER2 with ab11710 at 1/100 dilution, followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing strong membranous staining in SK-BR-3 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 diliution (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1: ab11710 at 1/100 dilution followed by ab150080 at a 1/1000 dilution.
Negative control 2: ab179513 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.
Negative control cells: MCF7 (PMID: 18288420).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide ab11710.
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![Flow Cytometry - Anti-ErbB2 / HER2 antibody [ICR12] - BSA and Azide free (ab256130)](https://www.abcam.com/ps/products/256/ab256130/Images/ab256130-4-anti-erbb-2-antibody-icr12-flow-cytometry-mcf7.jpg)
Flow Cytometry - Anti-ErbB2 / HER2 antibody [ICR12] - BSA and Azide free (ab256130)Flow cytometric analysis of MCF7 (human breast adenocarcinoma epithelial cell, Left panel) / SK-BR-3 (human breast adenocarcinoma epithelial cell, Right panel) cells labelling ErbB2 / HER2 with ab11710 at 1/1000 dilution (0.1µg) (Red) compared with a rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rat IgG (Alexa Fluor® 488, ab150157) at 1/2000 dilution was used as the secondary antibody.
Low expression control: MCF7. (PMID: 17938260).
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide ab11710.
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![Anti-ErbB2 / HER2 antibody [ICR12] - BSA and Azide free (ab256130)](https://www.abcam.com/ps/products/256/ab256130/Images/ab256130-5-benefits-of-recombinant-antibodies.png)
Anti-ErbB2 / HER2 antibody [ICR12] - BSA and Azide free (ab256130)