Anti-Sonic Hedgehog antibody (ab73958)
Key features and details
- Rabbit polyclonal to Sonic Hedgehog
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Sonic Hedgehog antibody
See all Sonic Hedgehog primary antibodies -
Description
Rabbit polyclonal to Sonic Hedgehog -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human
-
Immunogen
-
Positive control
- Human lung lysate and fetal brain lysate
-
General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sonic Hedgehog antibody (ab73958)
Human lung tissue stained with ab73958 at 1/100 dilution for 30 mins at RT.
Tissue sections boiled in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. -
Immunocytochemistry/ Immunofluorescence - Anti-Sonic Hedgehog antibody (ab73958)ICC/IF image of ab73958 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73958, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
