Anti-TXNRD1 antibody (ab113904)
Key features and details
- Rabbit polyclonal to TXNRD1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TXNRD1 antibody
See all TXNRD1 primary antibodies -
Description
Rabbit polyclonal to TXNRD1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in Human Testis and Spleen tissue lysates as well as the following whole cell lysates: HeLa; HepG2; HEK293; MCF7.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications CowDogPigChimpanzeeMacaque monkeyGorillaOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 71 kDa). Abcam recommends using milk as the blocking agent (3%).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 71 kDa). Abcam recommends using milk as the blocking agent (3%).Target
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Function
Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid. -
Tissue specificity
Isoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver. -
Sequence similarities
Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
Contains 1 glutaredoxin domain. -
Domain
The N-terminal glutaredoxin domain found in isoform 1 does not contain the C-P-Y-C redox-active motif normally found in glutaredoxins and has been found to be inactive in classical glutaredoxin assays. -
Post-translational
modificationsThe N-terminus of isoform 5 is blocked.
ISGylated. -
Cellular localization
Cytoplasm and Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 282388 Cow
- Entrez Gene: 7296 Human
- Entrez Gene: 396681 Pig
- Omim: 601112 Human
- SwissProt: O62768 Cow
- SwissProt: Q16881 Human
- SwissProt: Q9MYY8 Pig
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Alternative names
- cytoplasmic antibody
- Gene associated with retinoic and IFN-induced mortality 12 protein antibody
- Gene associated with retinoic and interferon-induced mortality 12 protein antibody
see all
Images
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Western blot - Anti-TXNRD1 antibody (ab113904)All lanes : Anti-TXNRD1 antibody (ab113904) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 150 kDa, 37 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
The predicted molecular weight of TXNRD1 is 71 kDa (SwissProt), however we expect to observe a banding pattern around 60 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Datasheets and documents
References (0)
ab113904 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-TXNRD1 antibody (ab113904)All lanes : Anti-TXNRD1 antibody (ab113904) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Human spleen tissue lysate - total protein (ab29699)
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 71 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 150 kDa, 37 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
The predicted molecular weight of TXNRD1 is 71 kDa (SwissProt), however we expect to observe a banding pattern around 60 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
