• Product name

  Recombinant human GLO1 protein
  See all GLO1 proteins and peptides

• Biological activity

  Specific activity: >0.4 units/mg (please enquire for specific batch value). One unit will form 1.0 µmol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 7.5, at 25°C. Specific activity was expressed in units/mg of protein.

• Purity

  > 90 % SDS-PAGE.
  Purified by using conventional chromatography techniques.
  

• Expression system

  Escherichia coli

• Protein length

  Full length protein

• Animal free

  No

• Nature

  Recombinant

• • Species

    Human

  • Sequence

    MAEPQPPSGG LTDEAALSCC SDADPSTKDF LLQQTMLRVK DPKKSLDFYT RVLGMTLIQK CDFPIMKFSL YFLAYEDKND IPKEKDEKIA WALSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKM ATLM

• Final assay concentrations:In 1.5ml reaction mix, the final concentrations are 79 mM potassium phosphate, 0.033%(w/v) reduced glutathine, 0.003% (w/v) bovine serum albumin, 0.033% methylglyoxal(w/v) and recombinant gyloxalase l (0.5ug, 1ug, 2ug).
• 1. Prepare protein-free reaction buffer without recombinant protein into suitable container (for each protein concentration).
• 2. Equilibrate protein-free reaction buffer to 25°C and monitor at A240nm (absorbance) until the value is constant, using a spectrophotometer.
• 3. Dilute various concentrations of recombinant gyloxalase l (0.5ug, 1ug, 2ug) in 50ul of 10mM potassium phosphate buffer with 0.1% (wv) BSA, pH7.4.
• Add 50ul of recombinant glyoxalase I solution to 1.45ml reaction buffer.
• Immediately mix by inversion and record the increase at A240nm for 5 minutes