Antibodies, Proteins, Kits and Reagents for Life Science

281 433 ₸ Product size

100 µl 281 433 ₸

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Anti-ENO1 antibody [EPR19758] (ab227978)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19758] to ENO1
Suitable for: IHC-P, WB, ICC/IF, Flow Cyt, IP
Reacts with: Mouse, Rat, Human, African green monkey

Product name

Anti-ENO1 antibody [EPR19758]
See all ENO1 primary antibodies
Description

Rabbit monoclonal [EPR19758] to ENO1
Host species

Rabbit
Specificity

ab227978 does not cross-react with ENO2 or ENO3.

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Mouse Human
IHC-P	Mouse Rat Human
IP	Human
WB	Mouse Rat African green monkey

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: MCF7, RAW 264.7, Jurkat, HeLa, NIH/3T3 and C6 whole cell lysates; human skeletal muscle tissue lysate; mouse brain, heart and kidney tissue lysates; rat brain, heart, liver and spleen tissue lysates. IP: Jurkat whole cell lysate. IHC-P: Human pancreas and clear cell renal cell carcinoma tissue; mouse and rat pancreas tissue. ICC/IF: HeLa and NIH/3T3 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19758
Isotype

IgG
Research areas

Western blot - Anti-ENO1 antibody [EPR19758] (ab227978)

All lanes : Anti-ENO1 antibody [EPR19758] (ab227978) at 1/1000 dilution

Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rat heart tissue lysate
Lane 6 : Rat liver tissue lysate
Lane 7 : Rat spleen tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 47 kDa
Observed band size: 47 kDa

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [EPR19758] (ab227978)

Immunofluorescent analysis of 4% paraformaldegyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ENO1 with ab227978 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weakly nuclear staining in HeLa cell line.

The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

-ve control: PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 antibody [EPR19758] (ab227978)

Immunohistochemical analysis of paraffin-embedded human clear cell renal cell carcinoma tissue labeling ENO1 with ab227978 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and nuclear staining in human clear cell kidney carcinoma (PMID: 26037892) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Flow Cytometry - Anti-ENO1 antibody [EPR19758] (ab227978)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ENO1 with ab227978 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue).

Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-ENO1 antibody [EPR19758] (ab227978)

ENO1 was immunoprecipitated from 0.35 mg of Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab227978 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227978 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: Jurkat whole cell lysate 10 μg (Input).

Lane 2: ab227978 IP in Jurkat whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227978 in Jurkat whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 antibody [EPR19758] (ab227978)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling ENO1 with ab227978 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and nuclear staining in medium-small ducts of human pancreas (PMID: 19425054) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 antibody [EPR19758] (ab227978)

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling ENO1 with ab227978 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and nuclear staining in medium-small ducts of mouse pancreas is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 antibody [EPR19758] (ab227978)

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling ENO1 with ab227978 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and nuclear staining in medium-small ducts of rat pancreas is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-ENO1 antibody [EPR19758] (ab227978)

All lanes : Anti-ENO1 antibody [EPR19758] (ab227978) at 1/1000 dilution

Lane 1 : Human skeletal muscle tissue lysate at 20 µg
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
Lane 5 : C6 (rat glial tumor glial cell line) whole cell lysate at 10 µg
Lane 6 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Lane 7 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 47 kDa
Observed band size: 47 kDa

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lane 1: 15 seconds.

Lanes 2-7: 1 second.
Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [EPR19758] (ab227978)

Immunofluorescent analysis of 4% paraformaldegyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells labeling ENO1 with ab227978 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and nuclear staining in NIH/3T3 cell line.

The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

-ve control: PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluo^r® 488) (ab150077) secondary antibody at 1/1000 dilution .
Anti-ENO1 antibody [EPR19758] (ab227978)

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