Mouse FSTL1/FRP ELISA Kit (ab277437)
Key features and details
- Sensitivity: 0.43 ng/ml
- Range: 0.41 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse FSTL1/FRP ELISA Kit
See all FSTL1/FRP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% General Inter-assay Sample n Mean SD CV% General -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.43 ng/ml -
Range
0.41 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 76.47 72% - 85% Plasma 78.87 70% - 104% Cell culture media 81.04 70% - 97% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse FSTL1/FRP ELISA Kit (ab277437) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse FSTL1/FRP in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Mouse FSTL1/FRP coated on a 96-well plate. Standards and samples are pipetted into the wells and Mouse FSTL1/FRP present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Mouse FSTL1/FRP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Mouse FSTL1/FRP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated Anti-Mouse FSTL1/FRP Antibody 2 vials Anti-Mouse FSTL1 FRP coated Microplate 1 unit Mouse FSTL1/FRP standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
May modulate the action of some growth factors on cell proliferation and differentiation. Binds heparin. -
Tissue specificity
Overexpressed in synovial tissues from rheumatoid arthritis. -
Sequence similarities
Contains 2 EF-hand domains.
Contains 1 follistatin-like domain.
Contains 1 Kazal-like domain.
Contains 1 VWFC domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Anti FSTL1
- FLJ50214
- FLJ52277
see all -
Database links
- Entrez Gene: 14314 Mouse
- SwissProt: Q62356 Mouse
- Unigene: 182434 Mouse
Images
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Mouse FSTL1/FRP ELISA kit (ab277437) Standard curve.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.