Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RAB8A antibody [MJF-R22] - BSA and Azide free (ab238651)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [MJF-R22] to RAB8A - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RAB8A antibody [MJF-R22] - BSA and Azide free
    See all RAB8A primary antibodies

  • Description

    Rabbit monoclonal [MJF-R22] to RAB8A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant full length protein corresponding to Human RAB8A aa 1 to the C-terminus. Antibody epitope is C-terminal amino acids KITPDQQ (AA190-196)
    Database link: P61006

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: A549 cell lysate, MCF7, NIH/3T3, PC-12 and C6 whole cell lysates.

  • General notes

    ab238651 is the carrier-free version of ab237702 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab238651 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody was developed with support from The Michael J. Fox Foundation.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-RAB8A antibody [MJF-R22] - BSA and Azide free (ab238651)
    Immunoprecipitation - Anti-RAB8A antibody [MJF-R22] - BSA and Azide free (ab238651)

    RAB8A was immunoprecipitated from varying amounts of MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate with 1 μg of ab237702 per IP test. Western blot was perfromed from the immunoprecipitate using ab237702 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/2000 dilution.

    Lane 1: MEF whole cell lysate (input).
    Lane 2-7: ab237702 IP in different amounts of MEF lysates from 25 µg to 800 µg whole cell lysate.
    Lane 8: Pre-immune IgG instead of ab237702 in MEF whole cell lysate.

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting Buffer and concentration: 5% BSA/TBST.

    Exposure time: 10 seconds.

    This image is kindly provided by our collaborator Dr. Dario Alessi, University of Dundee.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab237702).

  • Immunoprecipitation - Anti-RAB8A antibody [MJF-R22] - BSA and Azide free (ab238651)
    Immunoprecipitation - Anti-RAB8A antibody [MJF-R22] - BSA and Azide free (ab238651)

    RAB8A was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate using ab237702 at 1/30. Western blot was performed from the immunoprecipitate using ab237702 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

    Lane 1: NIH/3T3 whole cell lysate 10μg (Input).
    Lane 2: ab237702 IP in NIH/3T3 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237702 in NIH/3T3 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 5 seconds.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab237702).

  • Immunoprecipitation - Anti-RAB8A antibody [MJF-R22] - BSA and Azide free (ab238651)
    Immunoprecipitation - Anti-RAB8A antibody [MJF-R22] - BSA and Azide free (ab238651)

    RAB8A was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell line) whole cell lysate using ab237702 at 1/30. Western blot was performed from the immunoprecipitate using ab237702 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

    Lane 1: A549 (Human lung carcinoma epithelial cell) whole cell lysate 10μg (Input).
    Lane 2: ab237702 IP in A459 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237702 in A549 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 seconds.

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab237702).

  • Western blot - Anti-RAB8A antibody [MJF-R22] - BSA and Azide free (ab238651)
    Western blot - Anti-RAB8A antibody [MJF-R22] - BSA and Azide free (ab238651)
    All lanes : Anti-RAB8A antibody [MJF-R22] (ab237702) at 1/1000 dilution

    Lane 1 : Wild-type A549 (human lung carcinoma epithelial cell) whole cell lysate
    Lane 2 : Rab8A knockout A549 whole cell lysate
    Lane 3 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 4 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 5 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 6 : C6 (rat glial tumor glial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 24 kDa



    Blocking/Diluting buffer and concentration: 5% NFDM/TBST 

    Exposure time: 10 seconds

    The band around 50 kDa is unspecific band.

    The WT and Rab8A KO A549 lysates were kindly provided by our collaborator Dr. Dario Alessi, University of Dundee.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab237702).

  • Anti-RAB8A antibody [MJF-R22] - BSA and Azide free (ab238651)
    Anti-RAB8A antibody [MJF-R22] - BSA and Azide free (ab238651)

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