Anti-Fibronectin antibody [Fn-3] (ab18265)
![Anti-Fibronectin antibody [Fn-3] (ab18265)](https://www.abcam.com/ps/products/18/ab18265/Images/ab18265-27924-anti-fibronectin-antibody-fn-3-immunofluorescence.jpg "Anti-Fibronectin antibody [Fn-3] (ab18265)")
Key features and details
- Mouse monoclonal [Fn-3] to Fibronectin
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Fibronectin antibody [Fn-3]
See all Fibronectin primary antibodies -
Description
Mouse monoclonal [Fn-3] to Fibronectin -
Host species
Mouse -
Specificity
Reacts with human cellular fibronectin, but not plasma fibronectin. -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Human fibronectin expressed by simian virus 40 transformed human mammary epithelial cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
Fn-3 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [Fn-3] (ab18265)
ICC/IF image of ab18265 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18265, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
