Anti-Laminin antibody (ab11575)
Key features and details
- Rabbit polyclonal to Laminin
- Suitable for: Dot blot, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Laminin antibody
See all Laminin primary antibodies -
Description
Rabbit polyclonal to Laminin -
Host species
Rabbit -
Specificity
In dot blot immunoassay this antibody does not react with Fibronectin, Vitronectin, Collagen IV, or Chondroitin sulfate types A, B, and C. -
Tested Applications & Species
See all applications and species dataApplication Species Dot MouseIHC-P Human
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Immunogen
Full length native protein (purified) corresponding to Laminin. The immunogen is laminin isolated from the basement membrane of Englebreth Holm-Swarm (EHS) mouse sarcoma.
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General notes
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.027% PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (ab11575)Immunohistochemical analysis of formalin fixed paraffin-embedded human tongue sections labeling Laminin with ab11575 at 1/30 dilution. Goat Anti-Rabbit IgG, FITC conjugate was used as secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (ab11575)Immunohistochemical analysis of formalin fixed paraffin-embedded human tonsil sections labeling Laminin with ab11575 at 1/50 dilution. Goat Anti-Rabbit IgG, Cy3™ conjugate was used as secondary antibody.
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Dot Blot - Anti-Laminin antibody (ab11575)Immunoblotting
Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane was separated on SDS-PAGE and probed with Rabbit Anti-Laminin ab11575. The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase secondary antibody and a chemiluminescent substrate.
Lanes
1. antibody dilution 1/1,000
2. antibody dilution 1/2,000
3. antibody dilution 1/4,000
4. antibody dilution 1/8,000
5. antibody dilution 1/16,000
6. antibody dilution 1/32,000
7. Negative control: without primary antibody -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Laminin antibody (ab11575)This image shows formalin fixed paraffin embedded human skin stained for Laminin (proteinase K digestion, anti-laminin 1:200 – 30 minutes RT). The picture was kindly supplied as part of the review submitted by Elizabeth Chlipala.