Anti-Laminin beta 1 antibody [EPR23265-32] - BSA and Azide free (ab273053)
![Anti-Laminin beta 1 antibody [EPR23265-32] - BSA and Azide free (ab273053)](https://www.abcam.com/ps/products/273/ab273053/Images/ab273053-2-anti-laminin-beta-1-antibody-epr2326532-immunocytochemistry-hepg2.jpg "Anti-Laminin beta 1 antibody [EPR23265-32] - BSA and Azide free (ab273053)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23265-32] to Laminin beta 1 - BSA and Azide free
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-Laminin beta 1 antibody [EPR23265-32] - BSA and Azide free
See all Laminin beta 1 primary antibodies -
Description
Rabbit monoclonal [EPR23265-32] to Laminin beta 1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- ICC/IF: NIH/3T3 and HepG2 cells.
-
General notes
ab273053 is the carrier-free version of ab256380. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23265-32 -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Laminin beta 1 antibody [EPR23265-32] - BSA and Azide free (ab273053)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling Laminin beta 1 with ab256380 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HepG2 cell line ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256380).
-
![Immunocytochemistry/ Immunofluorescence - Anti-Laminin beta 1 antibody [EPR23265-32] - BSA and Azide free (ab273053)](https://www.abcam.com/ps/products/273/ab273053/Images/ab273053-1-anti-laminin-beta-1-antibody-epr2326532-immunocytochemistry-nih3t3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Laminin beta 1 antibody [EPR23265-32] - BSA and Azide free (ab273053)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 cells labelling Laminin beta 1 with ab256380 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in NIH/3T3 cell line ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab256380).
-
![Anti-Laminin beta 1 antibody [EPR23265-32] - BSA and Azide free (ab273053)](https://www.abcam.com/ps/products/273/ab273053/Images/ab273053-3-benefits-of-recombinant-antibodies.png)
Anti-Laminin beta 1 antibody [EPR23265-32] - BSA and Azide free (ab273053)
