Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [Ng2] to Neurogranin - BSA and Azide free
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Neurogranin antibody [Ng2] - BSA and Azide free
    See all Neurogranin primary antibodies

  • Description

    Mouse monoclonal [Ng2] to Neurogranin - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
    Unsuitable for: IHC or IHC-Fr

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • WB: Human brain tissue lysate; Mouse hippicampus tissue lysate; Rat hippocampus tissue lysate. IHC-P: Human, mouse and rat cerebrum tissue. IP: Mouse hippocampus tissue lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab273464 is the carrier-free version of ab273443. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab273464 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)
    Western blot - Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)
    All lanes : Anti-Neurogranin antibody [Ng2] (ab273443) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Mouse hippocampus tissue lysate
    Lane 3 : Mouse spleen tissue lysate
    Lane 4 : Rat hippocampus tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 8 kDa
    Observed band size: 15 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab273443, the same antibody clone in a different buffer formulation.

    Negative control: Mouse spleen (PMID: 20665622).
    The molecular weight observed is consistent with what has been described in the literature ( PMID: 20875798).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 10 secs; Lanes 2-4: 3 secs.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)

    This data was developed using ab273443, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Neurogranin with ab273443 at 1/20000 dilution (0.053µg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on human cerebrum tissue is observed. The section was incubated with ab273443 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins.

  • Immunoprecipitation - Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)
    Immunoprecipitation - Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)

    This data was developed using ab273443, the same antibody clone in a different buffer formulation.

    Neurogranin was immunoprecipitated from 0.35 mg mouse hippocampus tissue lysate with ab273443 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273443 at 1/1000 dilution. Mouse IgG for IP (HRP) (ab131368) was used at 1/10000 dilution.

    Lane 1: Mouse hippocampus tissue lysate 10µg.

    Lane 2: ab273443 IP in mouse hippocampus tissue lysate.

    Lane 3: Mouse monoclonal IgG2b instead of ab273443 in Mouse hippocampus tissue lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 58 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)

    This data was developed using ab273443, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Neurogranin with ab273443 at 1/20000 dilution (0.053µg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on mouse cerebrum tissue is observed. The section was incubated with ab273443 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)

    This data was developed using ab273443, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Neurogranin with ab273443 at 1/20000 dilution (0.053µg/ml) followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on rat cerebrum tissue is observed. The section was incubated with ab273443 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 10 mins.

  • Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)
    Anti-Neurogranin antibody [Ng2] - BSA and Azide free (ab273464)

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