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Signal Transduction Cytoskeleton / ECM Cytoskeleton Motor Proteins Myosin

Anti-MYL12A (phospho T17 + S18) antibody [EPR1457] (ab126739)

Price and availability

244 579 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-MYL12A (phospho T17 + S18) antibody [EPR1457] (ab126739)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1457] to MYL12A (phospho T17 + S18)
  • Suitable for: WB
  • Reacts with: Mouse

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Overview

  • Product name

    Anti-MYL12A (phospho T17 + S18) antibody [EPR1457]
    See all MYL12A primary antibodies
  • Description

    Rabbit monoclonal [EPR1457] to MYL12A (phospho T17 + S18)
  • Host species

    Rabbit
  • Specificity

    This antibody only detects MYL12A phosphorylated at Threonine 17 and Serine 18.
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human MYL12A (phospho T17 + S18).
    Database link: P19105

  • Positive control

    • NIH/3T3 cell lysate.
  • General notes

     

     

    Previously labelled as MRCL3.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR1457
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Myosin

Images

  • Western blot - Anti-MYL12A (phospho T17 + S18) antibody [EPR1457] (ab126739)
    Western blot - Anti-MYL12A (phospho T17 + S18) antibody [EPR1457] (ab126739)
    All lanes : Anti-MYL12A (phospho T17 + S18) antibody [EPR1457] (ab126739) at 1/1000 dilution

    Lane 1 : NIH/3T3 cell
    lysate
    Lane 2 : NIH/3T3 cell
    lysate + Calyculin A

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 20 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?

  • Anti-MYL12A (phospho T17 + S18) antibody [EPR1457] (ab126739)
    Anti-MYL12A (phospho T17 + S18) antibody [EPR1457] (ab126739)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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    Applications: WB

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    Applications: ICC/IF, WB

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