Anti-COX2 / Cyclooxygenase 2 antibody (ab52237)
Key features and details
- Rabbit polyclonal to COX2 / Cyclooxygenase 2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-COX2 / Cyclooxygenase 2 antibody
See all COX2 / Cyclooxygenase 2 primary antibodies -
Description
Rabbit polyclonal to COX2 / Cyclooxygenase 2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human COX2/ Cyclooxygenase 2.
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Positive control
- WB: Human primary myometrial cells whole cell lysate, A549 cells. ICC/IF: A549 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-COX2 / Cyclooxygenase 2 antibody (ab52237)Anti-COX2 / Cyclooxygenase 2 antibody (ab52237) at 1/1000 dilution + Human primary myometrial cells whole cell lysate at 20 µg
Secondary
Anti-rabbit IgG, HRP-linked at 1/4000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
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Western blot - Anti-COX2 / Cyclooxygenase 2 antibody (ab52237)All lanes : Anti-COX2 / Cyclooxygenase 2 antibody (ab52237) at 1/500 dilution
Lane 1 : A549 (Human lung adenocarcinoma epithelial)
Lane 2 : A549 (Human lung adenocarcinoma epithelial) with Synthesized Peptide
Predicted band size: 69 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-COX2 / Cyclooxygenase 2 antibody (ab52237)Immunocytochemistry/immunofluorescence analysis of A549 (Human lung adenocarcinoma epithelial) cells labelling COX2 / Cyclooxygenase 2 with ab52237 at dilution of 1/100. No staining was abserved when blocked with synthesized peptide as shown in the inset.
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Western blot - Anti-COX2 / Cyclooxygenase 2 antibody (ab52237) This image is courtesy of an anonymous AbreviewAnti-COX2 / Cyclooxygenase 2 antibody (ab52237) (Human mesothelioma whole cell lysate.)
Secondary
Goat anti-rabbit HRP
Predicted band size: 69 kDa
Please see accompanying abreview for additional information
