Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸ 10 µl 120 614 ₸

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Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (ab177300)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17897] to Histone H3 (mono methyl K122)
Suitable for: PepArr, WB
Reacts with: Mouse, Human

Product name

Anti-Histone H3 (mono methyl K122) antibody [EPR17897]
See all Histone H3 primary antibodies
Description

Rabbit monoclonal [EPR17897] to Histone H3 (mono methyl K122)
Host species

Rabbit
Tested Applications & Species

Application Species
PepArr
Human

WB
Mouse

Human

See all applications and species data
Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa and NIH/3T3 whole cell lysates; HeLa histone extraction lysate; HeLa lysate - G1/S arrested cells (thymidine treatment); HeLa lysate - G2/M arrested cells (sequential thymidine and nocodazole treatments).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
PepArr	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17897
Isotype

IgG
Research areas

Peptide Array - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (ab177300)

ab177300 was tested in Peptide array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).

Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.

The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.
Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (ab177300)

All lanes : Anti-ASPHD1 antibody - N-terminal (ab177330) at 1/5000 dilution

Lane 1 : HeLa cytosolic fraction
Lane 2 : HeLa nuclear fraction

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 30 seconds

Blocking and dilution buffer: 5% BSA/TBST.
Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (ab177300)

Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (ab177300) at 1/5000 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) histone extraction lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 15 seconds

Blocking/Dilution buffer: 5% BSA/TBST.
Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (ab177300)

Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (ab177300) at 1/5000 dilution + NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% BSA/TBST.
Western blot - Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (ab177300)

All lanes : Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (ab177300) at 1/5000 dilution

Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate: untreated, asynchronous cells
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate: G1/S arrested cells (thymidine treatment)
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate: G2/M arrested cells (sequential thymidine and nocodazole treatments)

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% BSA/TBST.
Anti-Histone H3 (mono methyl K122) antibody [EPR17897] (ab177300)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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