Anti-Histone H4 (phospho S1) antibody [RM194] (ab222769)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM194] to Histone H4 (phospho S1)
- Suitable for: WB, ICC/IF
- Reacts with: Human, Recombinant fragment
Overview
-
Product name
Anti-Histone H4 (phospho S1) antibody [RM194]
See all Histone H4 primary antibodies -
Description
Rabbit monoclonal [RM194] to Histone H4 (phospho S1) -
Host species
Rabbit -
Specificity
ab222769 reacts to Histone H4 phosphorylated at Serine 1. The reactivity is not affected by neighboring Arginine 3 modifications. No cross reactivity with Histone H2A phosphorylated at Serine 1. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human -
Immunogen
Synthetic peptide corresponding to Histone H4 (phospho S1).
-
Positive control
- WB: HeLa whole cell lysate. ICC/IF: HeLa cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
RM194 -
Isotype
IgG
Images
-
All lanes : Anti-Histone H4 (phospho S1) antibody [RM194] (ab222769) at 0.5 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Recombinant Histone H4
-
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H4 (red) using ab222769 (2 μg/mL) in ICC/IF.
Actin filaments were labeled with fluorescein phalloidin (green).
-