Anti-Histone H4 (propionyl K8) antibody (ab241116)
Key features and details
- Rabbit polyclonal to Histone H4 (propionyl K8)
- Suitable for: ChIP, WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H4 (propionyl K8) antibody
See all Histone H4 primary antibodies -
Description
Rabbit polyclonal to Histone H4 (propionyl K8) -
Host species
Rabbit -
Tested applications
Suitable for: ChIP, WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Histone H4 (propionyl K8).
Database link: P62805 -
Positive control
- WB: HeLa, A549, HEK-293 and K562, treated with 30mM sodium propionate for 4hr, whole cell lysates. ICC/IF: HeLa cells (treated with 10mM sodium propionate for 4hr). IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Images
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Western blot - Anti-Histone H4 (propionyl K8) antibody (ab241116)All lanes : Anti-Histone H4 (propionyl K8) antibody (ab241116) at 1/2000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate, treated (+) with 30mM sodium propionate for 4hr
Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate, treated (+) with 30mM sodium propionate for 4hr
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 30mM sodium propionate for 4hr
Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate, treated (+) with 30mM sodium propionate for 4hr
Lane 5 : HeLa whole cell lysate, untreated (-)
Lane 6 : A549 whole cell lysate, untreated (-)
Lane 7 : HEK-293 whole cell lysate, untreated (-)
Lane 8 : K562 whole cell lysate, untreated (-)
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 11 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (propionyl K8) antibody (ab241116)HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H4 (propionyl K8) (green) using ab241116 at 1/25 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor®488-conjugated Goat Anti-Rabbit IgG(H+L).
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Immunoprecipitation - Anti-Histone H4 (propionyl K8) antibody (ab241116)Histone H4 (propionyl K8) was immunoprecipitated from 500 µg HeLa (humanepithelial cell line from cervix adenocarcinoma) whole cell lysate with ab241116 at 1/50 dilution.
Lane 1: Rabbit control IgG IP in HeLa whole cell lysate.
Lane 2: ab241116 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 20 µg (Input).
For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
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ChIP - Anti-Histone H4 (propionyl K8) antibody (ab241116)Chromatin Immunoprecipitation HeLa (Human epithelial cell line from cervix adenocarcinoma) (4 x 106, treated with 10mM sodium propionate for 4h) were treated with micrococcal nuclease, sonicated, and immunoprecipitated with 5 μg ab241116 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
