Anti-Histone H4 (formyl K77) antibody (ab241121)
Key features and details
- Rabbit polyclonal to Histone H4 (formyl K77)
- Suitable for: WB, ICC, ChIP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H4 (formyl K77) antibody
See all Histone H4 primary antibodies -
Description
Rabbit polyclonal to Histone H4 (formyl K77) -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC, ChIPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Histone H4 (formyl K77).
Database link: P62805 -
Positive control
- WB: A549, HEK-293 and K562, treated with 30mM sodium butyrate for 4hr, whole cell lysates. ICC: HeLa cells (treated with 30mM sodium butyrate for 4hr). ChIP: Chromatin prepared from HeLa cells (treated with 30mM sodium butyrate for 4hr).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Images
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Western blot - Anti-Histone H4 (formyl K77) antibody (ab241121)All lanes : Anti-Histone H4 (formyl K77) antibody (ab241121) at 1/100 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate, treated (+) with 30mM sodium butyrate for 4hr
Lane 4 : A549 whole cell lysate, untreated (-)
Lane 5 : HEK-293 whole cell lysate, untreated (-)
Lane 6 : K562 whole cell lysate, untreated (-)
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 11 kDa
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Immunocytochemistry - Anti-Histone H4 (formyl K77) antibody (ab241121)HeLa (human epithelial cell line from cervix adenocarcinoma) cells (treated with 30mM sodium butyrate for 4hr) stained for Histone H4 (formyl K77) using ab241121 at 1/30 dilution in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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ChIP - Anti-Histone H4 (formyl K77) antibody (ab241121)Chromatin Immunoprecipitation HeLa (Human epithelial cell line from cervix adenocarcinoma) (106, treated with 30mM sodium butyrate for 4h) were treated with micrococcal nuclease, sonicated, and immunoprecipitated with 5μg ab241121 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
