Anti-LZTFL1 antibody (ab236795)
Key features and details
- Rabbit polyclonal to LZTFL1
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LZTFL1 antibody
See all LZTFL1 primary antibodies -
Description
Rabbit polyclonal to LZTFL1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cynomolgus monkey, Orangutan
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Immunogen
Recombinant fragment corresponding to Human LZTFL1 aa 100 to the C-terminus.
Database link: Q9NQ48 -
Positive control
- WB: HEK-293T, HepG2, HeLa and U-251 MG whole cell lysates. IHC-P: Human testis tissue. ICC/IF: U-251 MG cells. IP: HepG2 whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LZTFL1 antibody (ab236795)All lanes : Anti-LZTFL1 antibody (ab236795) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : U-251 MG (human brain glioma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 35 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LZTFL1 antibody (ab236795)Paraffin-embedded human testicular tissue stained for LZTFL1 using ab236795 at 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-LZTFL1 antibody (ab236795)U-251 MG (human brain glioma cell line) cells stained for LZTFL1 (green) using ab236795 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
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Immunoprecipitation - Anti-LZTFL1 antibody (ab236795)LZTFL1 was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate with ab236795 at 1/200 dilution.
Lane 1: Rabbit control IgG IP in HepG2 whole cell lysate.
Lane 2: ab236795 IP in HepG2 whole cell extract.
Lane 3: HepG2 whole cell lysate (20 μg) Input.
For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody at 1/50000 dilution.
