Antibodies, Proteins, Kits and Reagents for Life Science

Anti-ATF2 (phospho T71) antibody [ATF2T71-G3] (ab278689)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [ATF2T71-G3] to ATF2 (phospho T71)
Suitable for: WB, Flow Cyt
Reacts with: Mouse, Human

Product name

Anti-ATF2 (phospho T71) antibody [ATF2T71-G3]
See all ATF2 primary antibodies
Description

Rabbit monoclonal [ATF2T71-G3] to ATF2 (phospho T71)
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human ATF2 (phospho T71). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P15336

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Positive control
- WB: HEK-293T (treated with UV) cell extract. Flow cyt: Jurkat cells (treated with anisomycin); NIH/3T3 cells (trested with UV and PMA).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Application	Species
Flow Cyt	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Concentration information loading...
Purity

Protein A/G purified
Clonality

Monoclonal
Clone number

ATF2T71-G3
Isotype

IgG
Light chain type

kappa
Research areas

Flow Cytometry - Anti-ATF2 (phospho T71) antibody [ATF2T71-G3] (ab278689)

Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with anisomycin (green) using ab278689 at 0.1µg/mL.
Western blot - Anti-ATF2 (phospho T71) antibody [ATF2T71-G3] (ab278689)

All lanes : Anti-ATF2 (phospho T71) antibody [ATF2T71-G3] (ab278689) at 0.1 µg/ml

Lane 1 : HEK-293T cell extract untreated
Lane 2 : HEK-293T cell extract treated with UV

Predicted band size: 55 kDa
Flow Cytometry - Anti-ATF2 (phospho T71) antibody [ATF2T71-G3] (ab278689)

Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) or untreated (red) or treated with anisomycin (green) using ab278689.
Flow Cytometry - Anti-ATF2 (phospho T71) antibody [ATF2T71-G3] (ab278689)

Flow cytometric analysis of NIH3T3 cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control (orange) or untreated (red) or treated with UV and PMA (green) using ab278689 at 0.1µg/mL.
Flow Cytometry - Anti-ATF2 (phospho T71) antibody [ATF2T71-G3] (ab278689)

Peptide blocking flow cytometric analysis of Jurkat cells secondary antibody only negative control (light blue) or untreated (red) or anisomycin-treated (green) or untreated and blocked with phospho-peptide (black) or anisomycin and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or anisomycin and blocked with non-phospho peptide (purple) using ab278689 at 0.1µg/mL.
Anti-ATF2 (phospho T71) antibody [ATF2T71-G3] (ab278689)

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