Anti-EPO-R antibody [EPR22366-40] - BSA and Azide free (ab245127)
![Anti-EPO-R antibody [EPR22366-40] - BSA and Azide free (ab245127)](https://www.abcam.com/ps/products/245/ab245127/Images/ab245127-1-244202IHC1.jpg "Anti-EPO-R antibody [EPR22366-40] - BSA and Azide free (ab245127)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22366-40] to EPO-R - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Mouse, Rat
Overview
-
Product name
Anti-EPO-R antibody [EPR22366-40] - BSA and Azide free
See all EPO-R primary antibodies -
Description
Rabbit monoclonal [EPR22366-40] to EPO-R - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Mouse
-
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- IHC-P: Mouse E14.5 liver tissue. Mouse and rat spleen tissue.
-
General notes
Ab245127 is the carrier-free version of ab244202. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab245127 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22366-40 -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] - BSA and Azide free (ab245127)
Immunohistochemical analysis of paraffin-embedded mouse E14.5 liver tissue labeling EPO-R with ab244202 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse E14.5 liver (PMID: 9808074) is observed. The section was incubated with ab244202 for 30mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab244202).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] - BSA and Azide free (ab245127)](https://www.abcam.com/ps/products/245/ab245127/Images/ab245127-2-244202IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] - BSA and Azide free (ab245127)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling EPO-R with ab244202 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on red pulp of the mouse spleen (PMID: 24283209) is observed. The section was incubated with ab244202 for 30mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab244202).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] - BSA and Azide free (ab245127)](https://www.abcam.com/ps/products/245/ab245127/Images/ab245127-3-244202IHC3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] - BSA and Azide free (ab245127)Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling EPO-R with ab244202 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on red pulp of the rat spleen (PMID: 24283209) is observed. The section was incubated with ab244202 for 30mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab244202).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] - BSA and Azide free (ab245127)](https://www.abcam.com/ps/products/245/ab245127/Images/ab245127-4-244202IHC4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] - BSA and Azide free (ab245127)Negative Control:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling EPO-R with ab244202 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). No staining on mouse liver (PMID: 15259024) is observed. The section was incubated with ab244202 for 30mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab244202).
-
![Anti-EPO-R antibody [EPR22366-40] - BSA and Azide free (ab245127)](https://www.abcam.com/ps/products/245/ab245127/Images/ab245127-5-benefits-of-recombinant-antibodies.png)
Anti-EPO-R antibody [EPR22366-40] - BSA and Azide free (ab245127)
