Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EPO-R antibody [EPR22366-40] - BSA and Azide free (ab245127)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22366-40] to EPO-R - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-EPO-R antibody [EPR22366-40] - BSA and Azide free
    See all EPO-R primary antibodies

  • Description

    Rabbit monoclonal [EPR22366-40] to EPO-R - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse E14.5 liver tissue. Mouse and rat spleen tissue.

  • General notes

    Ab245127 is the carrier-free version of ab244202. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab245127 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] - BSA and Azide free (ab245127)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] - BSA and Azide free (ab245127)

    Immunohistochemical analysis of paraffin-embedded mouse E14.5 liver tissue labeling EPO-R with ab244202 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse E14.5 liver (PMID: 9808074) is observed. The section was incubated with ab244202 for 30mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab244202).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] - BSA and Azide free (ab245127)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] - BSA and Azide free (ab245127)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling EPO-R with ab244202 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on red pulp of the mouse spleen (PMID: 24283209) is observed. The section was incubated with ab244202 for 30mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

    Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab244202).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] - BSA and Azide free (ab245127)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] - BSA and Azide free (ab245127)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling EPO-R with ab244202 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on red pulp of the rat spleen (PMID: 24283209) is observed. The section was incubated with ab244202 for 30mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

    Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab244202).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] - BSA and Azide free (ab245127)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPO-R antibody [EPR22366-40] - BSA and Azide free (ab245127)

    Negative Control:

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling EPO-R with ab244202 at 1/500 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). No staining on mouse liver (PMID: 15259024) is observed. The section was incubated with ab244202 for 30mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

    Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab244202).

  • Anti-EPO-R antibody [EPR22366-40] - BSA and Azide free (ab245127)
    Anti-EPO-R antibody [EPR22366-40] - BSA and Azide free (ab245127)

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