Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Dcp1a antibody [EPR13822] (ab183709)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13822] to Dcp1a
  • Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Dcp1a antibody [EPR13822]
    See all Dcp1a primary antibodies

  • Description

    Rabbit monoclonal [EPR13822] to Dcp1a

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK- 293, HeLa, and HepG2 whole cell lysate; IHC-P: Human kidney tissue; ICC/IF: HepG2 and HeLa cells; Flow Cyt: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Dcp1a antibody [EPR13822] (ab183709)
    Western blot - Anti-Dcp1a antibody [EPR13822] (ab183709)
    All lanes : Anti-Dcp1a antibody [EPR13822] (ab183709) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293 whole cell lysate
    Lane 2 : DCP1A knockout HEK-293 whole cell lysate
    Lane 3 : HepG2 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 63 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 3: Merged signal (red and green). Green - ab183709 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab183709 was shown to specifically react with DCP1A in wild-type HEK-293 cells as signal was lost in DCP1A  knockout cells. Wild-type and DCP1A  knockout samples were subjected to SDS-PAGE. Ab183709 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Dcp1a antibody [EPR13822] (ab183709)
    Western blot - Anti-Dcp1a antibody [EPR13822] (ab183709)
    Anti-Dcp1a antibody [EPR13822] (ab183709) at 1/10000 dilution + 293T cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 63 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-Dcp1a antibody [EPR13822] (ab183709)
    Immunocytochemistry/ Immunofluorescence - Anti-Dcp1a antibody [EPR13822] (ab183709)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Dcp1a with ab183709 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution (green). Counter stained with Dapi (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dcp1a antibody [EPR13822] (ab183709)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dcp1a antibody [EPR13822] (ab183709)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Dcp1a with ab183709 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Dcp1a antibody [EPR13822] (ab183709)
    Immunocytochemistry/ Immunofluorescence - Anti-Dcp1a antibody [EPR13822] (ab183709)

    ab183709 staining Dcp1a in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Control: PBS only

  • Western blot - Anti-Dcp1a antibody [EPR13822] (ab183709)
    Western blot - Anti-Dcp1a antibody [EPR13822] (ab183709)
    All lanes : Anti-Dcp1a antibody [EPR13822] (ab183709) at 1/2000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 63 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?

  • Flow Cytometry - Anti-Dcp1a antibody [EPR13822] (ab183709)
    Flow Cytometry - Anti-Dcp1a antibody [EPR13822] (ab183709)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Dcp1a with ab183709 at 1/150 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Anti-Dcp1a antibody [EPR13822] (ab183709)
    Anti-Dcp1a antibody [EPR13822] (ab183709)

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