Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸ 10 µl 117 264 ₸

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Anti-Dcp1a antibody [EPR13822] (ab183709)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13822] to Dcp1a
Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
Knockout validated
Reacts with: Human

Product name

Anti-Dcp1a antibody [EPR13822]
See all Dcp1a primary antibodies
Description

Rabbit monoclonal [EPR13822] to Dcp1a
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK- 293, HeLa, and HepG2 whole cell lysate; IHC-P: Human kidney tissue; ICC/IF: HepG2 and HeLa cells; Flow Cyt: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR13822
Isotype

IgG
Research areas

Western blot - Anti-Dcp1a antibody [EPR13822] (ab183709)

All lanes : Anti-Dcp1a antibody [EPR13822] (ab183709) at 1/1000 dilution

Lane 1 : Wild-type HEK-293 whole cell lysate
Lane 2 : DCP1A knockout HEK-293 whole cell lysate
Lane 3 : HepG2 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 63 kDa
Observed band size: 75 kDa
why is the actual band size different from the predicted?

Lanes 1 - 3: Merged signal (red and green). Green - ab183709 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab183709 was shown to specifically react with DCP1A in wild-type HEK-293 cells as signal was lost in DCP1A knockout cells. Wild-type and DCP1A knockout samples were subjected to SDS-PAGE. Ab183709 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Dcp1a antibody [EPR13822] (ab183709)

Anti-Dcp1a antibody [EPR13822] (ab183709) at 1/10000 dilution + 293T cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 63 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-Dcp1a antibody [EPR13822] (ab183709)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Dcp1a with ab183709 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution (green). Counter stained with Dapi (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dcp1a antibody [EPR13822] (ab183709)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Dcp1a with ab183709 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Dcp1a antibody [EPR13822] (ab183709)

ab183709 staining Dcp1a in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

Control: PBS only
Western blot - Anti-Dcp1a antibody [EPR13822] (ab183709)

All lanes : Anti-Dcp1a antibody [EPR13822] (ab183709) at 1/2000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 63 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Flow Cytometry - Anti-Dcp1a antibody [EPR13822] (ab183709)

Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Dcp1a with ab183709 at 1/150 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Anti-Dcp1a antibody [EPR13822] (ab183709)

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