Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CSNK2A1 antibody [EP1963Y] (ab76040)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1963Y] to CSNK2A1
  • Suitable for: WB, Flow Cyt, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CSNK2A1 antibody [EP1963Y]
    See all CSNK2A1 primary antibodies

  • Description

    Rabbit monoclonal [EP1963Y] to CSNK2A1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human CSNK2A1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P68400
    (Peptide available as ab203372)

  • Positive control

    • WB: HeLa and HCT116 cell lysate IHC-P Human breast carcinoma tissue

  • General notes

     This product was previously labelled as CKII alpha

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-CSNK2A1 antibody [EP1963Y] (ab76040)
    Western blot - Anti-CSNK2A1 antibody [EP1963Y] (ab76040)
    All lanes : Anti-CSNK2A1 antibody [EP1963Y] (ab76040) at 1/1000 dilution

    Lane 1 : Wild-type HCT116 cell lysate
    Lane 2 : CSNK2A1 knockout HCT116 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa



    Lanes 1-2: Merged signal (red and green). Green - ab76040 observed at 45 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab76040 Anti-CSNK2A1 antibody [EP1963Y] was shown to specifically react with CSNK2A1 in wild-type HCT116 cells. Loss of signal was observed when knockout cell line ab266864 (knockout cell lysate ab257401) was used. Wild-type and CSNK2A1 knockout samples were subjected to SDS-PAGE. ab76040 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Western blot - Anti-CSNK2A1 antibody [EP1963Y] (ab76040)
    Western blot - Anti-CSNK2A1 antibody [EP1963Y] (ab76040)
    All lanes : Anti-CSNK2A1 antibody [EP1963Y] (ab76040) at 1/500 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : CSNK2A1 knockout HAP1 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 45 kDa



    Lanes 1 - 4: Merged signal (red and green). Green – ab76040 observed at 45 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab76040 was shown to specifically react with CSNK2A1 when CCSNK2A1 knockout samples were used. Wild-type and CSNK2A1 knockout samples were subjected to SDS-PAGE. ab76040 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSNK2A1 antibody [EP1963Y] (ab76040)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSNK2A1 antibody [EP1963Y] (ab76040)

    Immunohistochemical analysis of CSNK2A1 in paraffin embedded human breast carcinoma tissue using ab76040 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-CSNK2A1 antibody [EP1963Y] (ab76040)
    Western blot - Anti-CSNK2A1 antibody [EP1963Y] (ab76040)
    Anti-CSNK2A1 antibody [EP1963Y] (ab76040) at 1/1000 dilution + HeLa cell lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

  • Flow Cytometry - Anti-CSNK2A1 antibody [EP1963Y] (ab76040)
    Flow Cytometry - Anti-CSNK2A1 antibody [EP1963Y] (ab76040)
    Overlay histogram showing Jurkat cells stained with ab76040 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76040, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Anti-CSNK2A1 antibody [EP1963Y] (ab76040)
    Anti-CSNK2A1 antibody [EP1963Y] (ab76040)

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