Anti-CSNK2A1 antibody (ab236664)
Key features and details
- Rabbit polyclonal to CSNK2A1
- Suitable for: IHC-P, WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CSNK2A1 antibody
See all CSNK2A1 primary antibodies -
Description
Rabbit polyclonal to CSNK2A1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Xenopus laevis
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Immunogen
Recombinant full length protein corresponding to Human CSNK2A1 aa 1 to the C-terminus.
Database link: P68400 -
Positive control
- WB: HeLa, HEK-293, HepG2, Raji and K562 whole cell lysates. IHC-P: Human brain and colon cancer tissues. ICC/IF: HepG2 cells. IP: HeLa whole cell lysate (ab150035).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CSNK2A1 antibody (ab236664)All lanes : Anti-CSNK2A1 antibody (ab236664) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 5 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 45 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-CSNK2A1 antibody (ab236664)HepG2 (human liver hepatocellular carcinoma cell line) cells labeling CSNK2A1 (green) using ab236664 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSNK2A1 antibody (ab236664)Paraffin-embedded human colon cancer tissue stained for CSNK2A1 using ab236664 at 1/300 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSNK2A1 antibody (ab236664)Paraffin-embedded human brain tissue stained for CSNK2A1 using ab236664 at 1/300 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
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Immunoprecipitation - Anti-CSNK2A1 antibody (ab236664)CSNK2A1 was immunooprecipitated from 500 μg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate using ab236664 at 1/200 dilution. For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 1: Rabbit control IgG instead of ab236664 in Hela whole cell lysate.
Lane 2: ab236664 IP in HeLa whole cell lysate.
Lane 3: 10 μg HeLa whole cell lysate (input)
