Anti-Hsp70 antibody (ab124208)
Key features and details
- Rabbit polyclonal to Hsp70
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Hsp70 antibody
See all Hsp70 primary antibodies -
Description
Rabbit polyclonal to Hsp70 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Rabbit, Cow, Dog, Pig, Chimpanzee, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to Human Hsp70 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab124300) -
Positive control
- This antibody gave a positive signal in rat liver, kidney and brain tissue lysates, and in the following whole cell lysates: HeLa; Jurkat; HEK293; HepG2; A431; HUVEC; PC3; PC12.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab124208 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 70 kDa). ICC/IF Use a concentration of 5 µg/ml. Target
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Relevance
Function: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific. -
Cellular localization
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. -
Database links
- Entrez Gene: 281825 Cow
- Entrez Gene: 403612 Dog
- Entrez Gene: 15511 Human
- Entrez Gene: 3303 Human
- Entrez Gene: 396906 Pig
- Entrez Gene: 24472 Rat
- Entrez Gene: 294254 Rat
- Omim: 140550 Human
see all -
Alternative names
- DnaK type molecular chaperone HSP70 1 antibody
- Epididymis secretory protein Li 103 antibody
- FLJ54303 antibody
see all
Images
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Western blot - Anti-Hsp70 antibody (ab124208)All lanes : Anti-Hsp70 antibody (ab124208) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 6 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
Lane 7 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute -
Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody (ab124208)ICC/IF image of ab124208 stained HepG2 cells. The cells were 4% formaldehdye fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab124208, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Anti-Hsp70 antibody (ab124208)All lanes : Anti-Hsp70 antibody (ab124208) at 1 µg/ml
Lane 1 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 2 : Liver (Rat) Tissue Lysate
Lane 3 : Kidney (Rat) Tissue Lysate
Lane 4 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 46 kDa, 55 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
Protocols
Datasheets and documents
References (0)
ab124208 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Hsp70 antibody (ab124208)All lanes : Anti-Hsp70 antibody (ab124208) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 6 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
Lane 7 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
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Immunocytochemistry/ Immunofluorescence - Anti-Hsp70 antibody (ab124208)ICC/IF image of ab124208 stained HepG2 cells. The cells were 4% formaldehdye fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab124208, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-Hsp70 antibody (ab124208)All lanes : Anti-Hsp70 antibody (ab124208) at 1 µg/ml
Lane 1 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 2 : Liver (Rat) Tissue Lysate
Lane 3 : Kidney (Rat) Tissue Lysate
Lane 4 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 46 kDa, 55 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
