Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GM130 antibody [EP892Y] - BSA and Azide free (ab215966)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP892Y] to GM130 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IP, ICC/IF, IHC-P, WB
  • Reacts with: Dog, Human, African green monkey

Overview

  • Product name

    Anti-GM130 antibody [EP892Y] - BSA and Azide free
    See all GM130 primary antibodies

  • Description

    Rabbit monoclonal [EP892Y] to GM130 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IP, ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Dog, Human, African green monkey
    Predicted to work with: Cow, MonkeyDoes not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, MCF7, MDCK(NBL-2), MDBK(BL-1) and COS-1 cell lysates. IHC-P: Human cervix carcinoma and liver tissues. ICC/IF: HeLa and MCF7 cells.

  • General notes

    ab215966 is the carrier-free version of ab52649.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - BSA and Azide free (ab215966)
    Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - BSA and Azide free (ab215966) Image from Henry S.C. et al PLoS One. 2014 Apr 21;9(4):e95021. doi: 10.1371/journal.pone.0095021. eCollection 2014.

    Effect of Irgm1 palmitoylation mutation on Golgi association

    Irgm1 KO MEF were transfected with plasmids expressing wild-type or mutant Irgm1 proteins, as indicated. The cells were exposed to 100 U/ml IFN-γ for 24 h, stained with anti-Irgm1 and anti-GM130 antibodies, and used for immunofluorescence analysis. The experiment was performed 3 times, with at least 20 cells analyzed per group in each experiment. (A) Shown are images from representative cells. The scale bar represents 20 µm. 

    Cells are 4% paraformaldehyde fixed, 0.2% saponin-permeabilized.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52649).

  • Flow Cytometry - Anti-GM130 antibody [EP892Y] - BSA and Azide free (ab215966)
    Flow Cytometry - Anti-GM130 antibody [EP892Y] - BSA and Azide free (ab215966)

    Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling GM130 (red) with ab52649 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52649).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GM130 antibody [EP892Y] - BSA and Azide free (ab215966)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GM130 antibody [EP892Y] - BSA and Azide free (ab215966)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labelling GM130 with purified ab52649 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52649).

  • Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - BSA and Azide free (ab215966)
    Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - BSA and Azide free (ab215966)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling GM130 with purified ab52649 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/50) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52649).

  • Immunoprecipitation - Anti-GM130 antibody [EP892Y] - BSA and Azide free (ab215966)
    Immunoprecipitation - Anti-GM130 antibody [EP892Y] - BSA and Azide free (ab215966)

    ab52649 (purified) at 1/20 immunoprecipitating GM130 in HeLa whole cell lysate.

    Lane 1 (input): HeLa whole cell lysate (10µg)

    Lane 2 (+): ab52649 + HeLa whole cell lysate (10µg).

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52649 in HeLa whole cell lysate.

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution.

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52649).

  • Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - BSA and Azide free (ab215966)
    Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - BSA and Azide free (ab215966) This image is courtesy of an Abreview submitted by Aaron Halpen.

    Unpurified ab52649 staining GM130 (magenta) in monkey kidney cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 3% BSA + 0.5% Triton X-100 for 45 minutes at 25°C. Samples were incubated with primary antibody (1/1500 in 3% BSA + 0.5% Triton X-100) for 45 minutes at 25°C. An Alexa Fluor® 647-conjugated donkey anti-rabbit IgG polyclonal (2 µg/ml) was used as the secondary antibody. Nuclei stained with Picogreen.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52649).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GM130 antibody [EP892Y] - BSA and Azide free (ab215966)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GM130 antibody [EP892Y] - BSA and Azide free (ab215966)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling GM130 with unpurified ab52649 at a dilution of 1/500.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52649).

  • Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - BSA and Azide free (ab215966)
    Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - BSA and Azide free (ab215966) This image is courtesy of an Abreview submitted by Vladimir Milenkovic.

    Unpurified ab52649 staining GM130 in human ARPE-19 cells by ICC/IF (immunocytochemistry/immunofluorescence). Cells were formaldehyde fixed, permeabilized by 0.5% TX-100 and blocked with 5% serum for 20 minutes at 25°C. The sample was incubated with the primary antibody (1/500 in 1% goat serum, 0.1%TX100, 1 x PBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit polyclonal (1/500) was used as the secondary.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52649).

  • Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - BSA and Azide free (ab215966)
    Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - BSA and Azide free (ab215966) This image is courtesy of an Abreview submitted by JL Balligand.

    Unpurified ab52649 staining GM130 in Bovine brain microvascular endothelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% saponin and blocked with 5% BSA for 90 minutes at 37°C. Samples were incubated with primary antibody (1/100 in 0.1% saponin + 1% BSA ) for 18 hours at 4°C. An undiluted Alexa Fluor® 568-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52649).

  • Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - BSA and Azide free (ab215966)
    Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody [EP892Y] - BSA and Azide free (ab215966)

    ICC/IF image of unpurified ab52946 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (unpurified ab52946, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52649).

  • Anti-GM130 antibody [EP892Y] - BSA and Azide free (ab215966)
    Anti-GM130 antibody [EP892Y] - BSA and Azide free (ab215966)

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