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Anti-GM130 antibody (ab32337)

Price and availability

268 032 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-GM130 antibody (ab32337)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to GM130
  • Suitable for: WB
  • Reacts with: Drosophila melanogaster
  • Isotype: IgG

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Overview

  • Product name

    Anti-GM130 antibody
    See all GM130 primary antibodies
  • Description

    Rabbit polyclonal to GM130
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Drosophila melanogaster
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to GM130 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab32336)

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Golgi
    • Signal Transduction
    • Protein Trafficking
    • Golgi Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human GM130 protein (ab131818)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Drosophila melanogaster
Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).

Target

  • Function

    Golgi auto-antigen; probably involved in maintaining cis-Golgi structure.
  • Sequence similarities

    Belongs to the GOLGA2 family.
  • Domain

    Extended rod-like protein with coiled-coil domains.
  • Cellular localization

    Golgi apparatus > Golgi stack membrane.
  • Target information above from: UniProt accession Q08379 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • 130 kDa cis Golgi matrix protein antibody
    • 130 kDa cis-Golgi matrix protein antibody
    • Cis golgi matrix protein GM130 antibody
    • GM130 antibody
    • Gm130 autoantigen antibody
    • GOGA2_HUMAN antibody
    • GOLGA 2 antibody
    • Golga2 antibody
    • Golgi autoantigen antibody
    • Golgi autoantigen golgin subfamily a 2 antibody
    • Golgi matrix protein GM130 antibody
    • Golgin 95 antibody
    • golgin A2 antibody
    • Golgin subfamily a 2 antibody
    • Golgin subfamily A member 2 antibody
    • Golgin-95 antibody
    • MGC20672 antibody
    • SY11 protein antibody
    see all

Images

  • Western blot - Anti-GM130 antibody (ab32337)
    Western blot - Anti-GM130 antibody (ab32337)
    Anti-GM130 antibody (ab32337) at 1 µg/ml + Drosophila S2 Lysate at 10 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 95 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (3)

    Publishing research using ab32337? Please let us know so that we can cite the reference in this datasheet.

    ab32337 has been referenced in 3 publications.

    • Wang J  et al. Exosome-Mediated Transfer of lncRNA HOTTIP Promotes Cisplatin Resistance in Gastric Cancer Cells by Regulating HMGA1/miR-218 Axis. Onco Targets Ther 12:11325-11338 (2019). PubMed: 31908497
    • Maier PJ  et al. Ischemia-like oxygen and glucose deprivation mediates down-regulation of cell surface ?-aminobutyric acidB receptors via the endoplasmic reticulum (ER) stress-induced transcription factor CCAAT/enhancer-binding protein (C/EBP)-homologous protein (CHOP). J Biol Chem 289:12896-907 (2014). ICC/IF . PubMed: 24668805
    • O'Sullivan NC  et al. Reticulon-like-1, the Drosophila orthologue of the Hereditary Spastic Paraplegia gene reticulon 2, is required for organization of endoplasmic reticulum and of distal motor axons. Hum Mol Genet 21:3356-65 (2012). ICC/IF ; Drosophila melanogaster . PubMed: 22543973

    Images

    • Western blot - Anti-GM130 antibody (ab32337)
      Western blot - Anti-GM130 antibody (ab32337)
      Anti-GM130 antibody (ab32337) at 1 µg/ml + Drosophila S2 Lysate at 10 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 95 kDa
      Observed band size: 100 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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