Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637)
Key features and details
- Rabbit polyclonal to GM130 - Drosophila Golgi/Cis-Golgi Marker
- Suitable for: ICC/IF, WB
- Reacts with: Human, Drosophila melanogaster
- Isotype: IgG
Overview
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Product name
Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker
See all GM130 primary antibodies -
Description
Rabbit polyclonal to GM130 - Drosophila Golgi/Cis-Golgi Marker -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human, Drosophila melanogaster -
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Fruit fly (Drosophila melanogaster) GM130.
Read Abcam's proprietary immunogen policy (Peptide available as ab32335.) -
Positive control
- Drosophila S2 Cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab30637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 1 µg/ml. WB Use a concentration of 0.1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa). Target
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Function
Golgi auto-antigen; probably involved in maintaining cis-Golgi structure. -
Sequence similarities
Belongs to the GOLGA2 family. -
Domain
Extended rod-like protein with coiled-coil domains. -
Cellular localization
Golgi apparatus > Golgi stack membrane. - Information by UniProt
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Database links
- Entrez Gene: 2801 Human
- Omim: 602580 Human
- SwissProt: Q08379 Human
- Unigene: 155827 Human
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Alternative names
- 130 kDa cis Golgi matrix protein antibody
- 130 kDa cis-Golgi matrix protein antibody
- Cis golgi matrix protein GM130 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637)This image is courtesy of Sean Munro, MRC Laboratory of Molecular BiologyThe image shows staining of Drosophila S2 cells using ab30637 to cis-Golgi/Golgi marker, GM130. For comparison, S2 cells were stained with an antibody to the trans-Golgi marker, GCC88. Gm130 stained the cis-Golgi region and appears to be a superior Golgi marker to GCC88.
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Western blot - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637)This image is courtesy of Sean Munro, MRC Laboratory of Molecular BiologyAll lanes : Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637) at 0.1 µg/ml
Lane 1 : Drosophila melanogaster S2 cell extract
Lane 2 : Drosophila melanogaster S2 cell extract + specific siRNA
Predicted band size: 95 kDa
Observed band size: 95 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637)Image from Zhang L et al, J Biol Chem. 2010 Jun 18;285(25):19491-501. Epub 2010 Apr 6, Fig 1. DOI 10.1074/jbc.M109.098145 June 18, 2010 The Journal of Biological Chemistry, 285, 19491-19501.ab30637 at a 1/100 dilution staining Golgi/Cis-Golgi in Drosophila S2R+ cells by Immunocytochemistry/ Immunofluorescence.
Expression of recombinant wild type pgant3 (pIB-pgant3-V5), pgant3m1 (pIB-m1-V5), and pgant3m2 (pIB-m2-V5) in Drosophila S2R+ cells. Cells were then stained for V5 and the Golgi marker GM130 revealing Golgi localization of wild type pgant3 and pgant3m1. -
Western blot - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637)All lanes : Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637) at 1 µg/ml
Lane 1 :Schneider L2 whole cell lysate (ab14893)
Lane 2 :Schneider L2 whole cell lysate (ab14893) with D. melanogaster GM130 peptide (ab32335) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 95 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.
Protocols
References (56)
ab30637 has been referenced in 56 publications.
- Wan Z et al. Mononuclear phagocyte system blockade improves therapeutic exosome delivery to the myocardium. Theranostics 10:218-230 (2020). PubMed: 31903116
- Mukherjee A et al. Microtubules originate asymmetrically at the somatic golgi and are guided via Kinesin2 to maintain polarity within neurons. Elife 9:N/A (2020). PubMed: 32657758
- Wells MB & Andrew DJ Anopheles Salivary Gland Architecture Shapes Plasmodium Sporozoite Availability for Transmission. mBio 10:N/A (2019). PubMed: 31387905
- Laffafian A & Tepass U Identification of Genes Required for Apical Protein Trafficking in Drosophila Photoreceptor Cells. G3 (Bethesda) 9:4007-4017 (2019). PubMed: 31649044
- Xiong L et al. ER complex proteins are required for rhodopsin biosynthesis and photoreceptor survival in Drosophila and mice. Cell Death Differ N/A:N/A (2019). PubMed: 31263175
Images
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Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637) This image is courtesy of Sean Munro, MRC Laboratory of Molecular BiologyThe image shows staining of Drosophila S2 cells using ab30637 to cis-Golgi/Golgi marker, GM130. For comparison, S2 cells were stained with an antibody to the trans-Golgi marker, GCC88. Gm130 stained the cis-Golgi region and appears to be a superior Golgi marker to GCC88.
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Western blot - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637) This image is courtesy of Sean Munro, MRC Laboratory of Molecular BiologyAll lanes : Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637) at 0.1 µg/ml
Lane 1 : Drosophila melanogaster S2 cell extract
Lane 2 : Drosophila melanogaster S2 cell extract + specific siRNA
Predicted band size: 95 kDa
Observed band size: 95 kDa
-
Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637) Image from Zhang L et al, J Biol Chem. 2010 Jun 18;285(25):19491-501. Epub 2010 Apr 6, Fig 1. DOI 10.1074/jbc.M109.098145 June 18, 2010 The Journal of Biological Chemistry, 285, 19491-19501.ab30637 at a 1/100 dilution staining Golgi/Cis-Golgi in Drosophila S2R+ cells by Immunocytochemistry/ Immunofluorescence.
Expression of recombinant wild type pgant3 (pIB-pgant3-V5), pgant3m1 (pIB-m1-V5), and pgant3m2 (pIB-m2-V5) in Drosophila S2R+ cells. Cells were then stained for V5 and the Golgi marker GM130 revealing Golgi localization of wild type pgant3 and pgant3m1. -
Western blot - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637)All lanes : Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637) at 1 µg/ml
Lane 1 :Schneider L2 whole cell lysate (ab14893)
Lane 2 :Schneider L2 whole cell lysate (ab14893) with D. melanogaster GM130 peptide (ab32335) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 95 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.
